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Background: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahía Blanca city, Argentina (sites S2/S3) and in the dike that supplies water to it (S1). Methods: Culture-dependent method, biochemical tests, and molecular method (16S rRNA sequencing gene) were [ver mas...]
dc.contributor.authorOriani, Alejandra Soledad
dc.contributor.authorMarfil, Maria Jimena
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorBaldini, Mónica Diana
dc.coverage.spatialBuenos Aires (province)
dc.date.accessioned2019-10-24T18:06:01Z
dc.date.available2019-10-24T18:06:01Z
dc.date.issued2019-06
dc.identifier.issn2212-5531
dc.identifier.otherhttps://doi.org/10.4103/ijmy.ijmy_39_19
dc.identifier.urihttp://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=2;spage=138;epage=145;aulast=Oriani
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6200
dc.description.abstractBackground: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahía Blanca city, Argentina (sites S2/S3) and in the dike that supplies water to it (S1). Methods: Culture-dependent method, biochemical tests, and molecular method (16S rRNA sequencing gene) were combined to detect and identify NTM. Results: NTM were isolated in 51.6% (64/124) of all the samples analyzed. Mycobacterium gordonae was the most frequently isolated organism (15/64) in all samples analyzed, followed by Mycobacterium peregrinum and Mycobacterium frederiksbergense. Significant differences were found in the residual chlorine values between sampling S2 and S3. In both sites, maximum counts were recorded but they did not correlate with low chlorine values. A concentration higher than 500 colony-forming unit/L of NTM was never found, which can be attributed to the negative effect caused by decontamination methods being a point to consider for the recovery of NTM. In 46.9% (30/64) of samples, both methods coincided in the identification, and the obtained sequences presented ≥99% identity. Identification at the species level was achieved in 50% (32/64) of the isolates. Nearly 17.2% (11/64) of the isolates showed a similarity <99%. Conclusions: It should be taken into account that sequencing of the 16S rRNA gene and biochemical tests are useful for the identification of several species, but it is necessary to incorporate other genes (hsp 65 and rpo B) to obtain accurate identification.es_AR
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherWolters Kluwer; Asian African Society of Mycobacteriologyes_AR
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.sourceInternational journal of mycobacteriology 8 (2): 138‑145. (14-Jun-2019)eng
dc.subjectDrinking Water Systemseng
dc.subjectRed de Agua Potablees_AR
dc.subjectIsolation Techniqueseng
dc.subjectTécnicas de Aislamientoes_AR
dc.subjectMycobacteriumes_AR
dc.subject.otherIdentification Methodseng
dc.subject.otherMétodos de identificaciónes_AR
dc.subject.otherBahía Blanca, Buenos Aireses_AR
dc.subject.otherNontuberculous Mycobacteriaeng
dc.titlePrevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentinaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Oriani, Alejandra Soledad. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentinaes_AR
dc.description.filFil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Baldini, Mónica Diana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentinaes_AR
dc.subtypecientifico


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