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Abstract
Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was [ver mas...]
dc.contributor.authorNahirñak, Vanesa
dc.contributor.authorAlmasia, Natalia Ines
dc.contributor.authorFernandez, Paula Virginia
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorEstevez, Jose Manuel
dc.contributor.authorCarrari, Fernando
dc.contributor.authorVazquez Rovere, Cecilia
dc.date.accessioned2019-10-22T12:05:18Z
dc.date.available2019-10-22T12:05:18Z
dc.date.issued2012-01
dc.identifier.issn0032-0889
dc.identifier.issn1532-2548
dc.identifier.otherhttps://doi.org/10.1104/pp.111.186544
dc.identifier.urihttp://www.plantphysiol.org/content/158/1/252
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6165
dc.description.abstractSnakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70% to 90% increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherAmerican Society of Plant Biologistses_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourcePlant Physiology 158 (1) : 252-263 (January 2012)es_AR
dc.subjectPapaes_AR
dc.subjectPotatoeseng
dc.subjectPéptidoses_AR
dc.subjectPeptideseng
dc.subjectMetabolismoes_AR
dc.subjectMetabolismeng
dc.subjectPared Celulares_AR
dc.subjectCell Wallseng
dc.subjectDivisión Celulares_AR
dc.subjectCell Divisioneng
dc.subjectAntimicrobianoses_AR
dc.subjectAntimicrobialseng
dc.subject.otherSnakin-1es_AR
dc.titlePotato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Compositiones_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Fernandez, Paula Virginia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Química de Biomoléculas; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentinaes_AR
dc.description.filFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.subtypecientifico


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