Show simple item record

resumen

Abstract
The essential gene YNL152w was previously found in a screen designed to isolate putative negative regulators of the S. cerevisiae Pkc1p pathway. Activity assays were performed with a lexA-RLM1-lacZ integrated reporter in different ynl152w truncated mutants. In contrast to the original screen, there were no differences or the activities were even lower in some mutants. To analyze the consequences of different expression levels, YNL152w was expressed under [ver mas...]
dc.contributor.advisorHeinisch, Jürgen (director)
dc.contributor.authorCiklic, Ivan Francisco
dc.date.accessioned2019-10-10T12:29:37Z
dc.date.available2019-10-10T12:29:37Z
dc.date.issued2007
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6081
dc.identifier.urihttps://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2007062910
dc.descriptionTesis para obtener el grado de Doctor en Biología/Química de la Universität Osnabrück, en 2007es_AR
dc.description.abstractThe essential gene YNL152w was previously found in a screen designed to isolate putative negative regulators of the S. cerevisiae Pkc1p pathway. Activity assays were performed with a lexA-RLM1-lacZ integrated reporter in different ynl152w truncated mutants. In contrast to the original screen, there were no differences or the activities were even lower in some mutants. To analyze the consequences of different expression levels, YNL152w was expressed under the control of the GAL1/10 promoter. Growth curves were performed under high, intermediate and low expression levels. Strikingly, both conditional strains were able to grow under repressing conditions. However, an aberrant morphology was found suggesting that the cells are indeed affected by low amounts of Ynl152w protein. A series of successive Ynl152wp C-terminal truncations was analyzed to determine cell viability and to investigate the function of the protein. Remarkably, about 2/3 of the protein were dispensable to confer viability. Microscopic analyses of constructs revealed an aberrant morphology characteristic of a cytokinesis defective mutant, with the appearance of swollen cells and formation of big aggregates. Interestingly, the phenotype was more pronounced in the larger truncations. Coherent with these results time-lapse experiments with a large truncated mutant showed a stabilization of the SH3 protein Hof1p at the bud neck. This protein is involved in septum formation and has been reported as a binding partner of YNL152w. The phenotypes observed in the truncated mutants could be attributed to the presence of 4 proline rich motifs. Such motifs have been reported to interact with SH3 domains. An internal deletion of an aspartate rich domain present in the Ynl152wp sequence also displayed a phenotype very similar to that of the largest truncations. Therefore, this domain may play an important role in Ynl152wp function.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherOsnabrück Universität, Alemaniaes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectSaccharomyces Cerevisiaees_AR
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectDivisión Celulares_AR
dc.subjectCell Divisioneng
dc.subjectCitoquininases_AR
dc.subjectCytokininseng
dc.subjectFenotiposes_AR
dc.subjectPhenotypeseng
dc.subject.otherGen YNL152wes_AR
dc.subject.otherGene YNL152wes_AR
dc.titleStudies on the essential YNL152w open reading frame in Saccharomyces cerevisiaees_AR
dc.typeinfo:ar-repo/semantics/tesis doctorales_AR
dc.typeinfo:eu-repo/semantics/doctoralThesises_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenEEA Mendozaes_AR
dc.description.filFil: Ciklic, Ivan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentinaes_AR
dc.subtypetesis


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record

info:eu-repo/semantics/openAccess
Except where otherwise noted, this item's license is described as info:eu-repo/semantics/openAccess