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Abstract
To evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In [ver mas...]
dc.contributor.authorSanchez, Gerardo
dc.contributor.authorGerhardt, Nadia
dc.contributor.authorSiciliano, Florencia
dc.contributor.authorVojnov, Adrián
dc.contributor.authorMalcuit, Isabelle
dc.contributor.authorMarano, María Rosa
dc.date.accessioned2019-06-14T11:31:43Z
dc.date.available2019-06-14T11:31:43Z
dc.date.issued2010
dc.identifier.issn0894-0282
dc.identifier.issn1943-7706
dc.identifier.otherhttp://dx.doi.org/10.1094/MPMI-23-4-0394
dc.identifier.urihttps://www.apsnet.org/publications/mpmi/2010/April/Pages/23_4_394.aspx
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5317
dc.description.abstractTo evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species--generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the avirulent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-1a in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hypersensitive resistance to PVX.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceMolecular Plant-Microbe Interactions 23 (4) : 394-405. (2010)es_AR
dc.subjectPapaes_AR
dc.subjectPotatoeseng
dc.subjectSolanum tuberosumes_AR
dc.subjectPotato Virus Aeng
dc.subjectÁcido Salicílicoes_AR
dc.subjectSalicylic Acideng
dc.titleSalicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosumes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA San Pedroes_AR
dc.description.filFil: Sánchez, Gerardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es_AR
dc.description.filFil: Gerhardt, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es_AR
dc.description.filFil: Siciliano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.description.filFil: Vojnov, Adrián. Fundación Pablo Cassará; Argentinaes_AR
dc.description.filFil: Malcuit, Isabelle. Algentech Limited; Reino Unido. Institute of Food Research; Reino Unidoes_AR
dc.description.filFil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes_AR
dc.subtypecientifico


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