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Abstract
The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 interacts with cellular PDZ proteins. P5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic C-termini. P7-1 and P7-2 also [ver mas...]
dc.contributor.authorMaroniche, Guillermo Andrés
dc.contributor.authorMongelli, Vanesa Claudia
dc.contributor.authorLlauger, Gabriela
dc.contributor.authorAlfonso, Victoria
dc.contributor.authorTaboga, Oscar Alberto
dc.contributor.authorDel Vas, Mariana
dc.date.accessioned2019-06-13T13:00:45Z
dc.date.available2019-06-13T13:00:45Z
dc.date.issued2012-09
dc.identifier.issn0042-6822
dc.identifier.otherhttps://doi.org/10.1016/j.virol.2012.04.016
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0042682212002115
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5308
dc.description.abstractThe in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 interacts with cellular PDZ proteins. P5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic C-termini. P7-1 and P7-2 also entered the nucleus and therefore, along with P5-2, could function as regulators of host gene expression. P6 located in the cytoplasm and in perinuclear cloud-like inclusions, was driven to P9-1 viroplasm-like structures and co-localized with P7-2, P10 and α-tubulin, suggesting its involvement in viroplasm formation and viral intracellular movement. Finally, P9-2 was N-glycosylated and located at the plasma membrane in association with filopodia-like protrusions containing actin, suggesting a possible role in virus cell-to-cell movement and spread.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceVirology 430 (2) : 81-89 (September 2012)es_AR
dc.subjectVirus de las Plantases_AR
dc.subjectPlant Viruseseng
dc.subjectEnfermedades de las Plantases_AR
dc.subjectPlant Diseaseseng
dc.subjectProteínases_AR
dc.subjectProteinseng
dc.subjectExperimentación in Vivoes_AR
dc.subjectIn Vivo Experimentationeng
dc.subject.otherVirus del Mal de Río Cuartoes_AR
dc.subject.otherMal de Río Cuarto viruseng
dc.titleIn vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functionses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Mongelli, Vanesa Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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