Mostrar el registro sencillo del ítem
resumen
Resumen
Foot and mouth disease is caused by a non-enveloped virus (FMDV), which disposes several antigenic sites at the surface of their capsid proteins. The most relevant and immunodominant antigenic site of FMDV (site A or AnSA) includes a key virus–cell interaction element (RGD motif) located in the Viral Protein 1 (VP1), more precisely at the GH loop. AnSA includes a set of overlapped and mainly linear epitopes, which are the main targets of the humoral
[ver mas...]
dc.contributor.author | Marrero Diaz De Villegas, Rubén | |
dc.contributor.author | Rodríguez Limardo, Ramiro | |
dc.contributor.author | Carrillo, Elisa Cristina | |
dc.contributor.author | Konig, Guido Alberto | |
dc.contributor.author | Turjanski, Adrián G. | |
dc.date.accessioned | 2019-06-12T12:58:07Z | |
dc.date.available | 2019-06-12T12:58:07Z | |
dc.date.issued | 2015-10 | |
dc.identifier.issn | 0022-1759 | |
dc.identifier.other | https://doi.org/10.1016/j.jim.2015.06.008 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0022175915300090 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/5301 | |
dc.description.abstract | Foot and mouth disease is caused by a non-enveloped virus (FMDV), which disposes several antigenic sites at the surface of their capsid proteins. The most relevant and immunodominant antigenic site of FMDV (site A or AnSA) includes a key virus–cell interaction element (RGD motif) located in the Viral Protein 1 (VP1), more precisely at the GH loop. AnSA includes a set of overlapped and mainly linear epitopes, which are the main targets of the humoral immune response. Taking advantage over specific structural features of the GH loop, we have evaluated the influence of every amino acid residue at AnSA in the interaction with 2 neutralizing antibodies by molecular modeling techniques. Additionally, we constructed diverse interaction complexes with multiple site A mutants and discussed about the structural influence of amino acidic insertions in such relevant antigenic site of FMDV. Our approach is in agreement with previous ELISA experiments and allows the understanding of how FMDV mutations may alter the interaction with different antibodies, as we can estimate the contribution of each amino acid to the interaction. Overall, our work contributes to the development of specific vaccination strategies for FMD control. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Elsevier | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Journal of Immunological Methods 425 : 51-57 (October 2015) | es_AR |
dc.subject | Fiebre Aftosa | es_AR |
dc.subject | Foot and Mouth Disease | eng |
dc.subject | Enfermedades de los Animales | es_AR |
dc.subject | Animal Diseases | eng |
dc.subject | Virus Fiebre Aftosa | es_AR |
dc.subject | Aphthovirus | eng |
dc.subject | Anticuerpos Monoclonales | es_AR |
dc.subject | Monoclonal Antibodies | eng |
dc.subject | Bioinformática | es_AR |
dc.subject | Bioinformatics | eng |
dc.title | A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Marrero Diaz De Villegas, Rubén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Rodríguez Limardo, Ramiro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Bioinformática Estructural; Argentina | es_AR |
dc.description.fil | Fil: Carrillo, Elisa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Turjanski, Adrian G. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina | es_AR |
dc.subtype | cientifico |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
common
-
Artículos científicos [372]