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Abstract
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of infected Sf-9 cells, even when ac109 knockout viruses displayed a single-cell infection phenotype. Moreover, confocal microscopy analysis revealed that budded virions can enter the cytoplasm but are unable to enter the cell nucleus. This [ver mas...]
dc.contributor.authorAlfonso, Victoria
dc.contributor.authorMaroniche, Guillermo Andrés
dc.contributor.authorReca, Sol Rita
dc.contributor.authorLopez, Maria Gabriela
dc.contributor.authorDel Vas, Mariana
dc.contributor.authorTaboga, Oscar Alberto
dc.date.accessioned2019-05-15T13:34:37Z
dc.date.available2019-05-15T13:34:37Z
dc.date.issued2012-09
dc.identifier.issn1932-6203
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0046146
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046146
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5117
dc.description.abstractThe Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of infected Sf-9 cells, even when ac109 knockout viruses displayed a single-cell infection phenotype. Moreover, confocal microscopy analysis revealed that budded virions can enter the cytoplasm but are unable to enter the cell nucleus. This defect could be repaired by complementing ac109 in trans. In addition, polyhedra of normal size could be detected in Sf-9 nuclei infected with ac109 knockout viruses. However, electron microscopy demonstrated that these occlusion bodies were empty. Altogether, these results indicate that ac109 is required for infectivity of both phenotypes of virus.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherPlos Onees_AR
dc.relationinfo:eu-repograntAgreement/INTA/AERG/232161/AR./Vacunas a subunidades: optimización de la presentación antigénica e inmunomodulación y de los métodos biotecnológicos de producciónes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourcePLoS ONE 7 (9) : e46146. (2012)es_AR
dc.subjectLepidopteraes_AR
dc.subjectAutographa californicaes_AR
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectFenotiposes_AR
dc.subjectPhenotypeseng
dc.subjectViruses_AR
dc.subjectViruseseng
dc.titleAcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progenyes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentinaes_AR
dc.description.filFil: Reca, Sol Rita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.es_AR
dc.description.filFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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