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resumen

Resumen
The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosphodiesther bonds, as immunostimulants. Liposomes (Lip) were prepared with dipalmitoylphosphatidylcholine, cholesterol and stearylamine by Ethanol Injection [ver mas...]
dc.contributor.authorReidel, Ivana Gabriela
dc.contributor.authorGarcia, Maria Ines
dc.contributor.authorGonzález, Verónica Doris Guadalupe
dc.contributor.authorGiorello, Antonella
dc.contributor.authorCalvinho, Luis Fernando
dc.contributor.authorGennaro, Ana Maria
dc.contributor.authorVeaute, Carolina Melania Isabel
dc.date.accessioned2019-05-03T13:33:58Z
dc.date.available2019-05-03T13:33:58Z
dc.date.issued2017-06
dc.identifier.issn2208-2085
dc.identifier.urihttps://gnpublication.org/index.php/ans/article/view/81/73
dc.identifier.urihttp://hdl.handle.net/20.500.12123/5024
dc.description.abstractThe development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosphodiesther bonds, as immunostimulants. Liposomes (Lip) were prepared with dipalmitoylphosphatidylcholine, cholesterol and stearylamine by Ethanol Injection method. Immune response was assessed by immunization of Balb/c mice with: Lip+BSA Lip+BSA+CpG, CpG+BSA or aluminium hydroxide (Al(OH)3+BSA). Liposomal formulations were able to induce high antibody levels. Lip+BSA+CpG led to higher IgG levels than Lip+BSA (p<0.05, Mann-Whitney) and elicited the highest IgG2a levels. All the formulations induced antigen specific cellular proliferation, without significant differences, meanwhile Lip+BSA+CpG produced the highest levels of IFN-γ. These results showed these liposomes are versatile vehicles to potentiate and target the immune system to vaccination, leading to high humoral and cellular immune responses.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherInternational Journal for Researches_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceInternational Journal For Research In Applied And Natural Science 3 (6) : 1-19 (June 2017)es_AR
dc.subjectVacunaes_AR
dc.subjectVaccineseng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectImmune Responseeng
dc.subjectCoadyuvanteses_AR
dc.subjectAdjuvantseng
dc.subjectRatónes_AR
dc.subjectMiceeng
dc.subjectLiposomas (organulos)es_AR
dc.subjectLiposomes (organelles)eng
dc.titleEffects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in micees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.es_AR
dc.description.filFil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.es_AR
dc.description.filFil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentinaes_AR
dc.description.filFil: Giorello, Antonella. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.es_AR
dc.description.filFil: Gennaro, Ana Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; Argentinaes_AR
dc.description.filFil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.es_AR
dc.subtypecientifico


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