Show simple item record

resumen

Abstract
Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium [ver mas...]
dc.contributor.authorVazquez, Cristina Lourdes
dc.contributor.authorLerner, Thomas R.
dc.contributor.authorKasmapour, Bahram
dc.contributor.authorPei, Gang
dc.contributor.authorGronow, Achim
dc.contributor.authorBianco, María Veronica
dc.contributor.authorBlanco, Federico Carlos
dc.contributor.authorBleck, Christopher K.E.
dc.contributor.authorGeffers, Robert
dc.contributor.authorBigi, Fabiana
dc.contributor.authorAbraham, Wolf-Rainer
dc.contributor.authorGutierrez, Maximiliano Gabriel
dc.date.accessioned2019-04-09T14:25:13Z
dc.date.available2019-04-09T14:25:13Z
dc.date.issued2014-09
dc.identifier.issn1462-5814
dc.identifier.otherhttps://doi.org/10.1111/cmi.12303
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4858
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12303#references-section
dc.description.abstractSome intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re‐infection and also with the specific expression of stress‐ and survival‐related genes. Our findings identify bacterial traits implicated in the establishment of long‐term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherWiley
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.sourceCellular microbiology 16 (9) : 1425-1440. (September 2014)eng
dc.subjectMycobacteriumes_AR
dc.subjectLong Term Experimentseng
dc.subjectExperimentos de Largo Plazoes_AR
dc.subjectIntracellular Fluideng
dc.subjectLíquido Intracellulares_AR
dc.subjectMycobacterium Bovises_AR
dc.subjectGlycolipidseng
dc.subjectGlicolípidoses_AR
dc.subjectSurvivaleng
dc.subjectSupervivenciaes_AR
dc.titleExperimental selection of long‐term intracellular mycobacteriaeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemaniaes_AR
dc.description.filFil: Lerner, Thomas R. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretañaes_AR
dc.description.filFil: Kasmapour, Bahram. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemaniaes_AR
dc.description.filFil: Pei, Gang. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemaniaes_AR
dc.description.filFil: Gronow, Achim. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemaniaes_AR
dc.description.filFil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Bleck, Christopher K.E. University of Basel. Centre for Cellular Imaging and NanoAnalytics, Structural Biology and Biophysics, Biozentrum, Mattenstrasse 26; Suizaes_AR
dc.description.filFil: Geffers, Robert. Helmholtz Centre for Infection Research. Research Group Genome Analytics; Alemaniaes_AR
dc.description.filFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Abraham, Wolf-Rainer. Helmholtz Centre for Infection Research. Research Group Chemical Microbiology; Alemaniaes_AR
dc.description.filFil: Gutierrez, Maximiliano Gabriel. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretañaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record