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Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium
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dc.contributor.author | Vazquez, Cristina Lourdes | |
dc.contributor.author | Lerner, Thomas R. | |
dc.contributor.author | Kasmapour, Bahram | |
dc.contributor.author | Pei, Gang | |
dc.contributor.author | Gronow, Achim | |
dc.contributor.author | Bianco, María Veronica | |
dc.contributor.author | Blanco, Federico Carlos | |
dc.contributor.author | Bleck, Christopher K.E. | |
dc.contributor.author | Geffers, Robert | |
dc.contributor.author | Bigi, Fabiana | |
dc.contributor.author | Abraham, Wolf-Rainer | |
dc.contributor.author | Gutierrez, Maximiliano Gabriel | |
dc.date.accessioned | 2019-04-09T14:25:13Z | |
dc.date.available | 2019-04-09T14:25:13Z | |
dc.date.issued | 2014-09 | |
dc.identifier.issn | 1462-5814 | |
dc.identifier.other | https://doi.org/10.1111/cmi.12303 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4858 | |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12303#references-section | |
dc.description.abstract | Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re‐infection and also with the specific expression of stress‐ and survival‐related genes. Our findings identify bacterial traits implicated in the establishment of long‐term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | Wiley | |
dc.rights | info:eu-repo/semantics/openAccess | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Cellular microbiology 16 (9) : 1425-1440. (September 2014) | eng |
dc.subject | Mycobacterium | es_AR |
dc.subject | Long Term Experiments | eng |
dc.subject | Experimentos de Largo Plazo | es_AR |
dc.subject | Intracellular Fluid | eng |
dc.subject | Líquido Intracellular | es_AR |
dc.subject | Mycobacterium Bovis | es_AR |
dc.subject | Glycolipids | eng |
dc.subject | Glicolípidos | es_AR |
dc.subject | Survival | eng |
dc.subject | Supervivencia | es_AR |
dc.title | Experimental selection of long‐term intracellular mycobacteria | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania | es_AR |
dc.description.fil | Fil: Lerner, Thomas R. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretaña | es_AR |
dc.description.fil | Fil: Kasmapour, Bahram. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania | es_AR |
dc.description.fil | Fil: Pei, Gang. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania | es_AR |
dc.description.fil | Fil: Gronow, Achim. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania | es_AR |
dc.description.fil | Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Bleck, Christopher K.E. University of Basel. Centre for Cellular Imaging and NanoAnalytics, Structural Biology and Biophysics, Biozentrum, Mattenstrasse 26; Suiza | es_AR |
dc.description.fil | Fil: Geffers, Robert. Helmholtz Centre for Infection Research. Research Group Genome Analytics; Alemania | es_AR |
dc.description.fil | Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Abraham, Wolf-Rainer. Helmholtz Centre for Infection Research. Research Group Chemical Microbiology; Alemania | es_AR |
dc.description.fil | Fil: Gutierrez, Maximiliano Gabriel. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretaña | es_AR |
dc.subtype | cientifico |
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