Mostrar el registro sencillo del ítem
resumen
Resumen
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and
[ver mas...]
dc.contributor.author | Araújo, Cristina Pires de | |
dc.contributor.author | Osório, Ana Luiza Alves Rosa | |
dc.contributor.author | Jorge, Klaudia dos Santos Goncalves | |
dc.contributor.author | Ramos, Carlos Alberto N. | |
dc.contributor.author | Filho, Antonio Francisco S. | |
dc.contributor.author | Vidal, Carlos Eugênio Soto | |
dc.contributor.author | Roxo, Eliana | |
dc.contributor.author | Nishibe, Christiane | |
dc.contributor.author | Almeida Junior, Nalvo Franco de | |
dc.contributor.author | Júnior, Antônio A. F. | |
dc.contributor.author | Silva, Marcio Roberto | |
dc.contributor.author | Neto, Jose Diomedes Barbosa | |
dc.contributor.author | Cerqueira, Valíria Duarte | |
dc.contributor.author | Zumarraga, Martin Jose | |
dc.contributor.author | Araujo, Flabio Ribeiro de | |
dc.date.accessioned | 2019-04-09T13:15:57Z | |
dc.date.available | 2019-04-09T13:15:57Z | |
dc.date.issued | 2014-03 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | https://doi.org/10.1371/journal.pone.0091023 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4855 | |
dc.identifier.uri | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091023 | |
dc.description.abstract | In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | PLOS | |
dc.rights | info:eu-repo/semantics/openAccess | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | PLoS ONE 9 (3) : e91023. (2014) | eng |
dc.subject | Mycobacterium Bovis | es_AR |
dc.subject | PCR | eng |
dc.subject | Tejidos Animales | es_AR |
dc.subject | Animal Tissues | eng |
dc.subject | Cattle | eng |
dc.subject | Ganado Bovino | es_AR |
dc.subject | DNA | eng |
dc.subject | ADN | es_AR |
dc.subject | Tuberculosis | es_AR |
dc.subject.other | TbD1 | eng |
dc.subject.other | Polymerase Chain Reaction | eng |
dc.title | Detection of Mycobacterium bovis in Bovine and Bubaline tissues using Nested-PCR for TbD1 | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Araújo, Cristina Pires de. Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-graduação em Ciência Animal; Brasil | es_AR |
dc.description.fil | Fil: Osório, Ana Luiza Alves Rosa. Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-graduação em Ciência Animal; Brasil | es_AR |
dc.description.fil | Fil: Jorge, Klaudia dos Santos Goncalves. Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-graduação em Ciência Animal; Brasil | es_AR |
dc.description.fil | Fil: Ramos, Carlos Alberto N. Bolsista DTI, CNPq; Brasil | es_AR |
dc.description.fil | Fil: Filho, Antonio Francisco S. Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-graduação em Ciência Animal; Brasil | es_AR |
dc.description.fil | Fil: Vidal, Carlos Eugênio Soto. Universidade Federal de Santa Maria. Programa de Pos-Graduación en Medicina Veterinaria; Brasil | es_AR |
dc.description.fil | Fil: Roxo, Eliana. Instituto Biológico de São Paulo; Brasil | es_AR |
dc.description.fil | Fil: Nishibe, Christiane. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasil | es_AR |
dc.description.fil | Fil: Almeida Junior, Nalvo Franco de. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasil | es_AR |
dc.description.fil | Fil: Júnior, Antônio A. F. Laboratório Nacional Agropecuário, Pedro Leopoldo; Brasil | es_AR |
dc.description.fil | Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil | es_AR |
dc.description.fil | Fil: Neto, Jose Diomedes Barbosa. Universidade Federal do Pará; Brasil | es_AR |
dc.description.fil | Fil: Cerqueira, Valíria Duarte. Universidade Federal do Pará; Brasil | es_AR |
dc.description.fil | Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil | es_AR |
dc.subtype | cientifico |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
common
-
Artículos científicos [373]