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Abstract
Background; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) [ver mas...]
dc.contributor.authorDe Haro, Luis Alejandro
dc.contributor.authorArellano, Sofía Maité
dc.contributor.authorNovak, Ondrej
dc.contributor.authorFeil, Regina
dc.contributor.authorDumon, Analia Delfina
dc.contributor.authorMattio, Maria Fernanda
dc.contributor.authorTarkowska, Danuse
dc.contributor.authorLlauger, Gabriela
dc.contributor.authorStrnad, Miroslav
dc.contributor.authorLunn, John Edward
dc.contributor.authorPearce, Stephen
dc.contributor.authorFigueroa, Carlos María
dc.contributor.authorDel Vas, Mariana
dc.date.accessioned2019-04-03T16:43:30Z
dc.date.available2019-04-03T16:43:30Z
dc.date.issued2019-03
dc.identifier.issn1471-2229
dc.identifier.otherhttps://doi.org/10.1186/s12870-019-1709-y
dc.identifier.urihttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-1709-y
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4806
dc.description.abstractBackground; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements. Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves. Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherBMCes_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNBIO/1131022/AR./Genómica funcional y biología de sistemas.es_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceBMC Plant Biology 19 : 112 (Marzo 2019)es_AR
dc.subjectSymptomses_AR
dc.subjectSíntomaseng
dc.subjectFijiviruses_AR
dc.subjectPlant Growth Substanceseng
dc.subjectSustancias de Crecimiento Vegetales_AR
dc.subjectReoviridaees_AR
dc.subjectTrehaloseeng
dc.subjectTrehalosaes_AR
dc.subjectWheateng
dc.subjectTrigoes_AR
dc.subject.otherMal de Río Cuarto viruseng
dc.subject.otherVirus del mal de Río Cuartoes_AR
dc.subject.otherPlant Hormoneseng
dc.subject.otherHormonas Vegetaleses_AR
dc.subject.otherSucrose Metabolismeng
dc.subject.otherMetabolismo de la Sacarosaes_AR
dc.titleMal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaveses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Arellano, Sofía Maité. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Novak, Ondrej. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checaes_AR
dc.description.filFil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; Alemaniaes_AR
dc.description.filFil: Dumon, Analia Delina Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Tarkowska, Danuse. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checaes_AR
dc.description.filFil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Strnad, Miroslav. Palacký University. Laboratory of Growth Regulators; República Checa. Institute of Experimental Botany Czech Academy of Sciences; República Checaes_AR
dc.description.filFil: Lunn, John Edward. Max Planck Institute of Molecular Plant Physiology; Alemaniaes_AR
dc.description.filFil: Pearce, Stephen. Colorado State University. Department of Soil and Crop Sciences; Estados Unidoses_AR
dc.description.filFil: Figueroa, Carlos María. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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