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Abstract
The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) [ver mas...]
dc.contributor.authorAmigo, Natalia
dc.contributor.authorMercado, Elsa Cristina
dc.contributor.authorBentancor, Adriana Beatriz
dc.contributor.authorSingh, Pallavi
dc.contributor.authorVilte, Daniel Alejandro
dc.contributor.authorGerhardt, Elizabeth
dc.contributor.authorZotta, Elsa
dc.contributor.authorIbarra, Cristina Adriana
dc.contributor.authorManning, Shannon D.
dc.contributor.authorLarzabal, Mariano
dc.contributor.authorCataldi, Angel Adrian
dc.date.accessioned2019-03-27T17:45:08Z
dc.date.available2019-03-27T17:45:08Z
dc.date.issued2015-06-01
dc.identifier.issn1932-6203
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0127710
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4764
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127710
dc.description.abstractThe hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades and the clade 8 strains were associated with most of the cases of severe disease. In this study, eight randomly selected isolates of EHEC O157:H7 from cattle in Argentina were studied as well as two human isolates. Four of them were classified as clade 8 through the screening for 23 SNPs; the two human isolates grouped in this clade as well, while two strains were closely related to strains representing clade 6. To assess the pathogenicity of these strains, we assayed correlates of virulence. Shiga toxin production was determined by an ELISA kit. Four strains were high producers and one of these strains that belonged to a novel genotype showed high verocytotoxic activity in cultured cells. Also, these clade 8 and 6 strains showed high RBC lysis and adherence to epithelial cells. One of the clade 6 strains showed stronger inhibition of normal water absorption than E. coli O157:H7 EDL933 in human colonic explants. In addition, two of the strains showing high levels of Stx2 production and RBC lysis activity were associated with lethality and uremia in a mouse model. Consequently, circulation of such strains in cattle may partially contribute to the high incidence of HUS in Argentina.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherPLOSes_AR
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.sourcePLoS ONE 10 (6) : e0127710. (June 1, 2015)eng
dc.subjectEscherichia Colies_AR
dc.subjectCattleeng
dc.subjectGanado Bovinoes_AR
dc.subjectFenotiposes_AR
dc.subjectPhenotypeseng
dc.subjectMolecular Geneticseng
dc.subjectGenética Moleculares_AR
dc.subjectColoneng
dc.subjectColon (Intestino)es_AR
dc.subject.otherHemolytic-uremic Syndromeeng
dc.subject.otherSíndrome Urémico Hemolíticoes_AR
dc.subject.otherArgentinaes_AR
dc.titleClade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypeseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentinaes_AR
dc.description.filFil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Microbiología; Argentinaes_AR
dc.description.filFil: Singh, Pallavi. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidoses_AR
dc.description.filFil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentinaes_AR
dc.description.filFil: Gerhardt, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentinaes_AR
dc.description.filFil: Zotta, Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentinaes_AR
dc.description.filFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentinaes_AR
dc.description.filFil: Manning, Shannon D. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidoses_AR
dc.description.filFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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