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Mostrando ítems 1-11 de 11
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Characterization of GASA-1, a new vaccine candidate antigen of Babesia bovis
Flores, Daniela; Rodriguez, Anabel Elisa; Tomazic, Mariela Luján; Torioni, Susana Marta; Echaide, Ignacio Eduardo; Zamorano, Patricia Ines; Langellotti, Cecilia Ana; Araujo, Flabio Ribeiro de; Rolls, Peter; Schnittger, Leonhard; Florin-Christensen, Mónica (Elsevier, 2020-11)Surface proteins bound to the cell membrane by glycosylphosphatidylinositol (GPI) anchors are considered essential for the survival of pathogenic protozoans. In the case of the tick-transmitted hemoparasite Babesia bovis, ... -
Comparación de métodos de extracción de ADN para mejorar el diagnóstico molecular de Cryptosporidium sp. en materia fecal de terneros = Comparison of DNA extraction methods to improve the molecular diagnosis of Cryptosporidium spp. from fecal samples of calves
Toledo, Jonathan; Lombardelli, Joaquín Andrés; Galarza, Roxana Ivon; Tiranti, Karina; Garro, Carlos Javier; Florin-Christensen, Mónica; Schnittger, Leonhard; Tomazic, Mariela Luján (Universidad de Morón, 2019-07)Cryptosporidium sp. es un parásito, protozoo que infecta a una gran variedad de hospedadores vertebrados. Entre las más de 30 especies validadas en el género, la especie zoonótica Cryptosporidium parvum es la principal ... -
Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
Tomazic, Mariela Luján; Rodriguez, Anabel Elisa; Lombardelli Joaquín Andrés; Poklepovich Caride, Tomás Javier; Garro, Carlos Javier; Galarza, Roxana Ivon; Tiranti, Karina; Florin-Christensen, Mónica; Schnittger, Leonhard (Elsevier, 2018-12)The apicomplexan protozoan Cryptosporidium parvum is an important causative agent of diarrhea of neonatal bovines. Vaccination has been proposed as an advantageous strategy against cryptosporidiosis of calves since besides ... -
Incomplete sterols and hopanoids pathways in ciliates: Gene loss and acquisition during evolution as a source of biosynthetic genes
Tomazic, Mariela Luján; Poklepovich Caride, Tomás Javier; Nudel, Berta Clara; Nusblat, Alejandro David (Elsevier, 2014-05)Polycyclic triterpenoids, such as sterols and hopanoids, are essential components of plasmatic membrane in eukaryotic organisms. Although it is generally assumed that ciliates do not synthesize sterols, and many of them ... -
Molecular characterization of Cryptosporidium isolates from calves in Argentina
Tomazic, Mariela Luján; Maidana, Jimena; Dominguez, Mariana Gabriela; Louge Uriarte, Enrique Leopoldo; Galarza, Roxana Ivon; Garro, Carlos Javier; Florin-Christensen, Monica; Schnittger, Leonhard (2013-12)Cryptosporidiosis is responsible for significant fatalities of neonatal calves, resulting in substantial economic loss in dairy farming in several countries. Additionally, the high shedding of environmentally resistant ... -
Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites
Tomazic, Mariela Luján; Marugán-Hernandez, Virginia; Rodriguez, Anabel Elisa (Frontiers Media, 2022-01)Parasites of the phylum Apicomplexa are the causative agents of important diseases such as malaria, toxoplasmosis or cryptosporidiosis in humans, and babesiosis and coccidiosis in animals. Whereas the first human recombinant ... -
Occurrence of Cryptosporidium and other enteropathogens and their association with diarrhea in dairy calves of Buenos Aires province, Argentina
Garro, Carlos Javier; Morici, Gabriel Edgardo; Tomazic, Mariela Luján; Vilte, Daniel Alejandro; Encinas, Micaela; Vega, Celina Guadalupe; Bok, Marina; Parreño, Viviana; Schnittger, Leonhard (Elsevier, 2021-04)Cryptosporidiosis of neonatal dairy calves causes diarrhea, resulting in important economic losses. In Argentina, prevalence values of Cryptosporidium spp. and other enteropathogens such as group A rotavirus (RVA), bovine ... -
Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires Province, Argentina
Garro, Carlos Javier; Morici, Gabriel Edgardo; Utges, María E.; Tomazic, Mariela Luján; Schnittger, Leonhard (2016)In order to determine the prevalence and risk factors for shedding of Cryptosporidium spp. in dairy calves, a cross-sectional study was carried out in the northeastern region of Buenos Aires Province, Argentina. Fecal ... -
Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina
Lombardelli, Joaquín Andrés; Tomazic, Mariela Luján; Schnittger, Leonhard; Tiranti, Karina (Springer, 2019-07)Cryptosporidiosis of calves is caused by the enteroprotozoan Cryptosporidium spp. The disease results in intense diarrhea of calves associated with substantial economic losses in dairy farming worldwide. The aim of this ... -
Use of Chelex-100 for the molecular diagnosisof five animal pathogens
Tomazic, Mariela Luján; Hamer, Micaela; Bustos, Carla Paola; Arregui, Matias Ezequiel; Ascencio, Mariano; Saraullo, Vanina Rosa; Watanabe, Olivia; Brihuega, Bibiana Felicitas; Rodriguez, Anabel Elisa; Grune Loffler, Sylvia (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2021-06)Molecular tools have improved conventional veterinary diagnosis. Acid nucleic extraction is a key step for downstream applications. This work aimed to compare the DNA extraction method Chelex-100 resin (M1) ... -
What Do We Know about Surface Proteins of Chicken Parasites Eimeria?
Britez, Jesica Daiana; Rodriguez, Anabel Elisa; Di Ciaccio, Lucia Soledad; Marugán-Hernandez, Virginia; Tomazic, Mariela Luján (MDPI, 2023-05)Poultry is the first source of animal protein for human consumption. In a changing world, this sector is facing new challenges, such as a projected increase in demand, higher standards of food quality and safety, and ...