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Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells

Abstract
Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus–oocyte complexes were exposed to 107 ​tissue culture-infective doses (TCID50)/mL of an ncp-BVDV strain during IVM (in vitro maturation). After that, [ver mas...]
Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus–oocyte complexes were exposed to 107 ​tissue culture-infective doses (TCID50)/mL of an ncp-BVDV strain during IVM (in vitro maturation). After that, cumulus cells and the ZP were removed by hyaluronidase and pronase treatment, respectively, and the percentages of oocytes with polar body were analyzed as a sign of nuclear maturation. After passage through cell culture, the virus was isolated from granulosa cells, ZP-free mature oocytes, and ZP-intact mature oocytes. These results were confirmed by reverse transcription–polymerase chain reaction. After consecutive washes, the virus remained associated with ZP-free oocytes, maintaining its replication and infectivity in permissive cells. Based on these findings, it is concluded that the classical viral isolation procedure has a predictive value to detect BVDV associated with ZP-free oocytes and that it was novelty demonstrated that both washing and trypsin treatment of oocytes were ineffective to remove BVDV infection. [Cerrar]
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Author
González Altamiranda, Erika Analía;   Kaiser, German Gustavo;   Rios, Glenda Laura;   Leunda, Maria Rosa;   Odeon, Anselmo Carlos;  
Fuente
Theriogenology 86 (8) : 1999-2003 (November 2016)
Date
2016-11
Editorial
Elsevier
ISSN
0093-691X
1879-3231
URI
https://www.sciencedirect.com/science/article/pii/S0093691X16302783
http://hdl.handle.net/20.500.12123/4570
DOI
https://doi.org/10.1016/j.theriogenology.2016.06.020
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Enfermedades de los Animales; Animal Diseases; Ganado Bovino; Cattle; Diarrea Viral Bovina; Bovine Viral Diarrhoea; Pestivirus de la Diarrea Bovina; Bovine Diarrhoea Pestivirus; Óvulo; Ova; Ovocitos;
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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