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Combined epidermal growth factor and hyaluronic acid supplementation of in vitro maturation medium and its impact on bovine oocyte proteome and competence
Rios, Glenda Laura; Buschiazzo, Jorgelina; Mucci, Nicolas Crescencio; Kaiser, German Gustavo; Cesari, Andreina; Alberio, Ricardo (Elsevier, 2015-03-15)The conditions for in vitro oocyte maturation impact on cytoplasmic and nuclear processes in the oocyte. These events are differentially influenced by the nature of the maturation inducer and the presence of intact cumulus ... -
Dataset fron: Binding and fusion of Hoechst-labelled sperm to ZP-free oocytes matured in the presence of 43 µM or 100 µM LA (fresh and vitrified) [Conjunto de datos]
Rios, Glenda Laura; Suqueli García, María Florencia; Manrique, Rodrigo J.; Buschiazzo, Jorgelina (Biotecnología de la Reproducción - Laboratorio de Producción in vitro de Embriones - IPADS Balcarce (INTA-CONICET), 2025)The file contains the folowing columns : FRESH: non-vitrified oocytes. VITRIFIED: oocytes vitrified using a surface device (Cryotech®). CONTROL : oocytes matured without LA. 43 µM LA: oocytes matured in 43 µM LA. ... -
Detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid: A potential risk for in vitro embryo production
Yavorsky, Marisol Solange; Norero, Natalia Sigrid; Spetter, Lucas Maximiliano; Pereyra, Susana Beatriz; Verna, Andrea Elizabeth; Urrutia Luna, Naiara; Rios, Glenda Laura; González Altamiranda, Erika (Elsevier, 2025-08)Bovine Viral Diarrhea Virus (BVDV) and anti-BVDV antibodies have been detected in ovarian follicular fluid of infected cows. The aim of this study was to assess both the frequency of BVDV detection and the prevalence of ... -
Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
González, Noelia; Gil, María Lydia; Rios, Glenda Laura; Canizo, Jésica Romina; De Blas, José Ignacio; Alberio, Ricardo (Bentham Open, 2014-02)The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from ... -
Effect of Seminal Plasma on Post‐Thaw Quality and Functionality of Corriedale Ram Sperm Obtained by Electroejaculation and Artificial Vagina
Ledesma, Alba; Manes, Jorgelina; Rios, Glenda Laura; Aller Atucha, Juan Florencio; Cesari, Andreina; Alberio, Ricardo; Hozbor, Federico Andres (Wiley, 2015-06)We have already shown that seminal collection method affects seminal plasma composition and sperm quality in Corriedale rams. In this study, we evaluated the effect of seminal plasma collected by electroejaculation or ... -
Exogenous progesterone during in vitro fertilization improves developmental competence of partially cumulus-denuded bovine oocytes
Suqueli García, María Florencia; Gabbanelli, Nadia; Rios, Glenda Laura (Elsevier, 2023-11)The progesterone (P4) secreted by cumulus cells has gained attention for its role as a possible physiological inducer of sperm acrosome exocytosis. In mammals, it is generally accepted that fertilization rates of oocytes ... -
Free cholesterol and cholesterol esters in bovine oocytes: Implications in survival and membrane raft organization after cryopreservation
Buschiazzo, Jorgelina; Rios, Glenda Laura; Canizo, Jésica Romina; Antollini, Silvia Susana; Alberio, Ricardo (2017)Part of the damage caused by cryopreservation of mammalian oocytes occurs at the plasma membrane. The addition of cholesterol to cell membranes as a strategy to make it more tolerant to cryopreservation has been little ... -
Improve intra‐uterine insemination in rabbits using ultra‐high temperature skim milk as extender to keep semen at room temperature
Hozbor, Federico Andres; Ledesma, Alba; Manes, Jorgelina; Rios, Glenda Laura; Kaiser, German Gustavo; Cano, Adriana Virginia; Luciano, Cecilia Ines; Alberio, Ricardo (Wiley, 2016-03)Two experiments were carried out to examine in vitro quality and in vivo fertility of rabbit semen diluted in ultra‐high temperature (UHT) skim milk. In the first experiment, pooled ejaculates of 10 adult rabbits were ... -
Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
González Altamiranda, Erika Analía; Kaiser, German Gustavo; Rios, Glenda Laura; Leunda, Maria Rosa; Odeon, Anselmo Carlos (Elsevier, 2016-11)Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility ... -
Linoleic acid stimulates neutral lipid accumulation in lipid droplets of maturing bovine oocytes
Carro, María De Las Mercedes; Buschiazzo, Jorgelina; Rios, Glenda Laura; Oresti, Gerardo Martin; Alberio, Ricardo (Elsevier, 2013-03-01)Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in bovine follicular fluid; when added to maturation culture media, it affects oocyte competence (depending on the type and concentration ... -
Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina
González Altamiranda, Erika Analía; Manrique, Julieta Marina; Perez, Sandra; Rios, Glenda Laura; Odeon, Anselmo Carlos; Leunda, Maria Rosa; Jones, Leandro Roberto; Verna, Andrea Elizabeth (Plos One, 2015-07-15)Bovine herpesvirus 4 (BoHV-4) is increasingly considered as responsible for various problems of the reproductive tract. The virus infects mainly blood mononuclear cells and displays specific tropism for vascular endothelia, ... -
Optimization of bovine oocyte cryopreservation: membrane fusion competence and cell death of linoleic acid-in vitro matured oocytes subjected to vitrification
Rios, Glenda Laura; Suqueli García, María Florencia; Manrique, Rodrigo J.; Buschiazzo, Jorgelina (Frontiers Media, 2025-07-03)Long-term conservation of livestock female genetic resources has led to increased interest in oocyte cryopreservation. However, the challenges of preserving large-volume cells and the lack of standardization in vitrification ... -
¿Por qué y para qué producir embriones bovinos in vitro?
Rios, Glenda Laura; Buschiazzo, Jorgelina (Estación Experimental Agropecuaria Balcarce, INTA, 2022-07)Nuevo récord mundial de embriones bovinos producidos in vitro promueve un giro hacia el uso de la criopreservación asociada al incremento sostenido en la producción de estos embriones. -
The effect of melatonin and season on in vivo embryo production of Dohne Merino ewes
Buffoni, Andres; Vozzi, Pedro Alejandro; Gonzalez, Diego Martin; Rios, Glenda Laura; Viegas Bordeira, Jorge Horacio; Abecia, José Alfonso (2014-07)To determine the effect of melatonin on embryo production in superovulated donor Dohne Merino ewes, two experiments were carried out at Trelew, Argentina (43° S 65° W), during the breeding and the anestrous seasons. ... -
Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa
Manes, Jorgelina; Rios, Glenda Laura; Fiorentino, María Andrea; Ungerfeld, Rodolfo (2016)The use of intravaginal sponges (IS) to synchronize estrous onset in ewes provokes vaginitis, an increase in the vaginal bacterial load, and growth of bacterial species that are not present during spontaneous estrous ...