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Abstract
Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two-component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional [ver mas...]
dc.contributor.authorGarcia, Elizabeth Andrea
dc.contributor.authorBlanco, Federico Carlos
dc.contributor.authorBigi, María Mercedes
dc.contributor.authorVazquez, Cristina Lourdes
dc.contributor.authorForrellad, Marina Andrea
dc.contributor.authorRocha, Rosana Valeria
dc.contributor.authorGolby, Paul
dc.contributor.authorSoria, Marcelo Abel
dc.contributor.authorBigi, Fabiana
dc.date.accessioned2019-03-01T14:49:02Z
dc.date.available2019-03-01T14:49:02Z
dc.date.issued2018-08
dc.identifier.issn0378-1135
dc.identifier.otherhttps://doi.org/10.1016/j.vetmic.2018.06.016
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4531
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0378113518300725?via%3Dihub
dc.description.abstractMycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two-component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional two-component system. In this study we investigated the role of PhoPR in two M. bovis strains with differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis. Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox balance.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElseviereng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceVeterinary microbiology 222 : 30-38. (August 2018)eng
dc.subjectMycobacterium Bovises_AR
dc.subjectComponentseng
dc.subjectGene Expressioneng
dc.subjectExpresión Génicaes_AR
dc.subject.otherPhoPReng
dc.titleCharacterization of the two component regulatory system PhoPR in Mycobacterium boviseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía, Cátedra de Microbiología Agrícola; Argentina. . Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentinaes_AR
dc.description.filFil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Rocha, Rosana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Golby, Paul. CN Bio Innovations, Welwyn Garden City; Reino Unidoes_AR
dc.description.filFil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentinaes_AR
dc.description.filFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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