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Abstract
Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic Escherichia coli (EPEC) are attaching and effacing (A/E) pathogens, which translocate effector proteins to intestinal enterocytes through a type III secretion system (T3SS). T3SS and most of its effector proteins are encoded in a pathogenicity island called LEE. Recently, new effectors have been located outside the LEE. This study aimed to characterize EspY3, a novel non-LEE encoded T3SS [ver mas...]
dc.contributor.authorLarzabal, Mariano
dc.contributor.authorMarques Da Silva, Wanderson
dc.contributor.authorRiviere, Nahuel Agustín
dc.contributor.authorCataldi, Angel Adrian
dc.date.accessioned2019-02-20T13:17:23Z
dc.date.available2019-02-20T13:17:23Z
dc.date.issued2018
dc.identifier.issn2076-2607
dc.identifier.otherhttps://doi.org/10.3390/microorganisms6040112
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4473
dc.identifier.urihttps://www.mdpi.com/2076-2607/6/4/112
dc.description.abstractEnterohemorrhagic Escherichia coli (EHEC) and enteropathogenic Escherichia coli (EPEC) are attaching and effacing (A/E) pathogens, which translocate effector proteins to intestinal enterocytes through a type III secretion system (T3SS). T3SS and most of its effector proteins are encoded in a pathogenicity island called LEE. Recently, new effectors have been located outside the LEE. This study aimed to characterize EspY3, a novel non-LEE encoded T3SS effector of EHEC. EspY3 shares homology with SopD and PipB2 effector proteins of Salmonella’s T3SS-1 and T3SS-2, respectively. The presence of recombinant EspY3 in the supernatant samples demonstrated that EspY3 was secreted by the T3SS of EHEC and EPEC. Through infection assays, we demonstrated the translocation of EspY3 into Caco-2 cells by T3SS of EPEC. The subcellular localization of EspY3 was determined in the pedestal region, where its presence generates a significant increase in the size of the pedestals area. The EspY3 effector induced the elongation of polymerized actin pedestals in infected Caco-2 by EPEC. This study confirmed that EspY3 is part of the repertoire of T3SS effectors of EHEC O157:H7, and that it participates in modeling cellular actin during the infectioneng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherMDPI AGeng
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.sourceMicroorganisms 6 (4) : 112. (2018)eng
dc.subjectEscherichia Colies_AR
dc.subjectActineng
dc.subjectActinaes_AR
dc.subjectPolymerizationeng
dc.subjectPolimerizaciónes_AR
dc.subjectEffectorseng
dc.subjectEfectores Moleculareses_AR
dc.subjectProteínases_AR
dc.subjectProteinseng
dc.subject.otherEspY3eng
dc.subject.otherT3SSeng
dc.subject.otherType III Secretion Systemeng
dc.titleNovel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestalseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Riviere, Nahuel Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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