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Abstract
Here, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB1–3, a polypeptide derived from non-structural proteins of foot-and-mouth disease virus, to polyhedrin (POLH), the major constituent of OBs, under polh promoter. To further assess the most convenient strategy to improve yields, we designed two recombinant baculoviruses, vPOLH and vPOLHE44G. These carried the sequence of [ver mas...]
dc.contributor.authorDiez, Michay
dc.contributor.authorTrotta, Myrian Vanesa
dc.contributor.authorAlfonso, Victoria
dc.contributor.authorTaboga, Oscar Alberto
dc.contributor.authorLopez, Maria Gabriela
dc.date.accessioned2019-02-18T16:28:56Z
dc.date.available2019-02-18T16:28:56Z
dc.date.issued2018-11
dc.identifier.issn2190-572X
dc.identifier.issn2190-5738 (Online)
dc.identifier.otherhttps://doi.org/10.1007/s13205-018-1482-x
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4459
dc.description.abstractHere, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB1–3, a polypeptide derived from non-structural proteins of foot-and-mouth disease virus, to polyhedrin (POLH), the major constituent of OBs, under polh promoter. To further assess the most convenient strategy to improve yields, we designed two recombinant baculoviruses, vPOLH and vPOLHE44G. These carried the sequence of the fusion protein POLH-Δ3AB1–3 with an additional copy in cis of polh or polhE44G, respectively, under p10 promoter. Our results show that both viruses expressed POLH-Δ3AB1–3, which was detected by western blot in purified rOBs with anti-POLH and anti-3AB1 antibodies. We also found that vPOLHE44G produced larger polyhedra and a significant increase of antigen yield (p < 0.01). Furthermore, the chimeric protein POLH-Δ3AB1–3 was recognized by sera from experimentally infected animals, showing that translational fusion to POLH does not alter the antigenicity of Δ3AB1–3. Finally, the rOBs were successfully used in an ELISA test to differentiate infected from vaccinated animals. Taken together, these results demonstrate the great potential of rOBs to develop diagnostic schemes adaptable to animal infectious diseases.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherSpringereng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.source3 Biotech 8 : 457. (November 2018)eng
dc.subjectBaculoviruses_AR
dc.subjectVirus Fiebre Aftosaes_AR
dc.subjectAphthoviruseng
dc.subjectELISAeng
dc.subject.otherOcclusion Bodieseng
dc.subject.otherCuerpos de Oclusiónes_AR
dc.subject.otherFoot and Mouth Disease Viruseng
dc.subject.otherPolyhedrineng
dc.subject.otherFMDVeng
dc.subject.otherDIVA Diagnosticeng
dc.titleRecombinant occlusion bodies of baculovirus as carriers of a nonstructural protein of foot-and-mouth disease viruseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Diez, Michay. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Trotta, Myrian Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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