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Resumen
Snakin-1 is a cysteine-rich antimicrobial peptide (AMP) isolated from potato tubers, with broad-spectrum activity.
It belongs to the Snakin/GASA family, whose members have been studied because of their diverse roles in
important plant processes, including defense. To analyze if this defensive function may lead to disease tolerance
in lettuce, one of the most worldwide consumed leafy vegetable, we characterized three homozygous transgenic
lines
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dc.contributor.author | Darqui, Flavia Soledad | |
dc.contributor.author | Radonic, Laura Mabel | |
dc.contributor.author | Trotz, Paulina M. | |
dc.contributor.author | Lopez, Nilda Ester | |
dc.contributor.author | Vazquez Rovere, Cecilia | |
dc.contributor.author | Hopp, Horacio Esteban | |
dc.contributor.author | Lopez Bilbao, Marisa Gisela | |
dc.date.accessioned | 2019-02-18T14:12:15Z | |
dc.date.available | 2019-02-18T14:12:15Z | |
dc.date.issued | 2018-10 | |
dc.identifier.issn | 0168-1656 | |
dc.identifier.other | https://doi.org/10.1016/j.jbiotec.2018.07.017 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4457 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0168165618305492?via%3Dihub#! | |
dc.description.abstract | Snakin-1 is a cysteine-rich antimicrobial peptide (AMP) isolated from potato tubers, with broad-spectrum activity. It belongs to the Snakin/GASA family, whose members have been studied because of their diverse roles in important plant processes, including defense. To analyze if this defensive function may lead to disease tolerance in lettuce, one of the most worldwide consumed leafy vegetable, we characterized three homozygous transgenic lines overexpressing Snakin-1. They were biologically assessed by the inoculation with the fungal pathogens Rhizoctonia solani and Sclerotinia sclerotiorum both in vitro and in planta at the greenhouse. When in vitro assays were performed with R. solani on Petri dishes containing crude plant extracts it was confirmed that the expressed Snakin-1 protein has antimicrobial activity. Furthermore, transgenic lines showed a better response than wild type in in vivo challenges against R. solani both in chamber and in greenhouse. In addition, two of these lines showed significant in vivo protection against the pathogen S. sclerotiorum in challenge assays on adult plants. Our results show that Snakin-1 is an interesting candidate gene for the selection/breeding of lettuce plants with increased fungal tolerance. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | Elsevier | eng |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Journal of biotechnology 283 : 62-69. (10 October 2018) | eng |
dc.subject | Rhizoctonia Solani | es_AR |
dc.subject | Sclerotinia Sclerotiorum | es_AR |
dc.subject | Resistencia a la Enfermedad | es_AR |
dc.subject | Disease Resistance | eng |
dc.subject | Lechugas | es_AR |
dc.subject | Lettuces | eng |
dc.subject | Transgénicos | es_AR |
dc.subject | Transgenics | eng |
dc.subject | Antimicrobial Agents | eng |
dc.subject | Agente Antimicrobiano | es_AR |
dc.subject | Hongos Patógenos | es_AR |
dc.subject | Pathogenic Fungi | eng |
dc.subject.other | Snakin-1 | eng |
dc.title | Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Trotz, Paulina M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Lopez, Nilda Ester. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina | es_AR |
dc.description.fil | Fil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.subtype | cientifico |
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