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Abstract
The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to [ver mas...]
dc.contributor.authorPeralta, Andrea Veronica
dc.contributor.authorMaroniche, Guillermo Andrés
dc.contributor.authorAlfonso, Victoria
dc.contributor.authorMolinari, Maria Paula
dc.contributor.authorTaboga, Oscar Alberto
dc.date.accessioned2019-01-30T12:12:29Z
dc.date.available2019-01-30T12:12:29Z
dc.date.issued2013-07
dc.identifier.issn0168-1702
dc.identifier.otherhttps://doi.org/10.1016/j.virusres.2013.03.018
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0168170213001123
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4355
dc.description.abstractThe Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVirus Research 175 (1) : 87-90 (July 2013)es_AR
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectVirus Fiebre Aftosaes_AR
dc.subjectAphthoviruseng
dc.subjectFiebre Aftosaes_AR
dc.subjectFoot and Mouth Diseaseeng
dc.subjectGanadoes_AR
dc.subjectLivestockeng
dc.subjectBaculoviruses_AR
dc.titleVP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface displayes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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