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Abstract
The endangered Cedrela balansae C.DC. (Meliaceae) is a high-value timber species with great potential for forest plantations that inhabits the tropical forests in Northwestern Argentina.Research on this species is scarce because of the limited genetic and genomic information available. Here, we explored the transcriptome of C. balansae using 454 GS FLX Titanium next-generation sequencing (NGS) technology. Following de novo assembling, we identified 27,111 [ver mas...]
dc.contributor.authorTorales, Susana Leonor
dc.contributor.authorRivarola, Maximo Lisandro
dc.contributor.authorGonzalez, Sergio Alberto
dc.contributor.authorInza, Maria Virginia
dc.contributor.authorPomponio, Maria Florencia
dc.contributor.authorFernandez, Paula Del Carmen
dc.contributor.authorAcuña, Cintia Vanesa
dc.contributor.authorZelener, Noga
dc.contributor.authorFornes, Luis Fernando
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorPaniego, Norma Beatriz
dc.contributor.authorMarcucci Poltri, Susana Noemi
dc.date.accessioned2019-01-23T16:45:27Z
dc.date.available2019-01-23T16:45:27Z
dc.date.issued2018-12
dc.identifier.issn1932-6203
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0203768
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0203768
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4320
dc.description.abstractThe endangered Cedrela balansae C.DC. (Meliaceae) is a high-value timber species with great potential for forest plantations that inhabits the tropical forests in Northwestern Argentina.Research on this species is scarce because of the limited genetic and genomic information available. Here, we explored the transcriptome of C. balansae using 454 GS FLX Titanium next-generation sequencing (NGS) technology. Following de novo assembling, we identified 27,111 non-redundant unigenes longer than 200 bp, and considered these transcripts for further downstream analysis. The functional annotation was performed searching the 27,111 unigenes against the NR-Protein and the Interproscan databases. This analysis revealed 26,977 genes with homology in at least one of the Database analyzed. Furthermore, 7,774 unigenes in 142 different active biological pathways in C. balansae were identified with the KEGG database. Moreover, after in silico analyses, we detected 2,663 simple sequence repeats (SSRs) markers. A subset of 70 SSRs related to important “stress tolerance” traits based on functional annotation evidence, were selected for wet PCR-validation in C. balansae and other Cedrela species inhabiting in northwest and northeast of Argentina (C. fissilis, C. saltensis and C. angustifolia). Successful transferability was between 77% and 93% and thanks to this study, 32 polymorphic functional SSRs for all analyzed Cedrela species are now available. The gene catalog and molecular markers obtained here represent a starting point for further research, which will assist genetic breeding programs in the Cedrela genus and will contribute to identifying key populations for its preservation.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherPlos Onees_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourcePLoS ONE 13 (12) : e0203768 (December 2018)es_AR
dc.subjectCedrelaes_AR
dc.subjectÁrboles Maderableses_AR
dc.subjectTimber Treeseng
dc.subjectMarcadores Genéticoses_AR
dc.subjectGenetic Markerseng
dc.subjectSecuencia Nucleotídicaes_AR
dc.subjectNucleotide Sequenceeng
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectBosque Primarioes_AR
dc.subjectPrimary Forestseng
dc.subject.otherCedrela balansaees_AR
dc.subject.otherMarcadores Moleculareses_AR
dc.subject.otherBosques Nativoses_AR
dc.subject.otherRegión Noroeste, Argentinaes_AR
dc.titleDe novo transcriptome sequencing and SSR markers development for Cedrela balansae C.DC., a native tree species of northwest Argentinaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Recursos Biológicoses_AR
dc.description.filFil: Torales, Susana Leonor. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentinaes_AR
dc.description.filFil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Inza, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentinaes_AR
dc.description.filFil: Pomponio, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentinaes_AR
dc.description.filFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Zelener, Noga. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentinaes_AR
dc.description.filFil: Fornes, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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