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IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis
Abstract
Protective immunity against Mycobacterium tuberculosis (Mtb) requires IFNG. Besides, IFNG-mediated induction of autophagy suppresses survival of virulent Mtb in macrophage cell lines. We investigated the contribution of autophagy to the defense against Mtb antigen (Mtb-Ag) in cells from tuberculosis patients and healthy donors (HD). Patients were classified as high responders (HR) if their T cells produced significant IFNG against Mtb-Ag; and low
[ver mas...]
Protective immunity against Mycobacterium tuberculosis (Mtb) requires IFNG. Besides, IFNG-mediated induction of autophagy suppresses survival of virulent Mtb in macrophage cell lines. We investigated the contribution of autophagy to the defense against Mtb antigen (Mtb-Ag) in cells from tuberculosis patients and healthy donors (HD). Patients were classified as high responders (HR) if their T cells produced significant IFNG against Mtb-Ag; and low responders (LR) when patients showed weak or no T cell responses to Mtb-Ag. The highest autophagy levels were detected in HD cells whereas the lowest quantities were observed in LR patients. Interestingly, upon Mtb-Ag stimulation, we detected a positive correlation between IFNG and MAP1LC3B-II/LC3-II levels. Actually, blockage of Mtb-Ag-induced IFNG markedly reduced autophagy in HR patients whereas addition of limited amounts of IFNG significantly increased autophagy in LR patients. Therefore, autophagy collaborates with human immune responses against Mtb in close association with specific IFNG secreted against the pathogen.
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![Thumbnail](/xmlui/bitstream/handle/20.500.12123/4303/Authophagy-cover.jpg?sequence=3&isAllowed=y)
Author
Rovetta, Ana Inés;
Peña, Delfina;
Hernández del Pino, Rodrigo Emanuel;
Recalde, Gabriela María;
Pellegrini, Joaquín Miguel;
Bigi, Fabiana;
Musella, Rosa María;
Palmero, Domingo;
Gutierrez, Marisa;
Colombo, Maria Isabel;
García, Verónica Edith;
Fuente
Autophagy 10 (12) : 2109-2121 (November 2014)
Date
2014-11
Editorial
Taylor & Francis
ISSN
1554-8627
1554-8635
1554-8635
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Derechos de acceso
Abierto
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