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Abstract
Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic [ver mas...]
dc.contributor.authorRomano, Nelson Gaston
dc.contributor.authorGioffre, Andrea
dc.contributor.authorSede, Silvana Mabel
dc.contributor.authorCampos, Eleonora
dc.contributor.authorCataldi, Angel Adrian
dc.contributor.authorTalia, Paola Mónica
dc.date.accessioned2019-01-15T17:55:21Z
dc.date.available2019-01-15T17:55:21Z
dc.date.issued2013-08
dc.identifier.issn0343-8651
dc.identifier.issn1432-0991
dc.identifier.otherhttps://doi.org/10.1007/s00284-013-0345-2
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00284-013-0345-2
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4272
dc.description.abstractCellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic conditions were characterized and compared among the three consortia. Thermal and pH stability were analyzed. Based on its cellulolytic activity, one consortium was selected for further characterization by zymography. We detected a specific protein of 55 kDa with CMCase activity. In this study, we have shown that these consortia encode for cellulolytic enzymes. These enzymes could be useful for lignocellulosic biomass degradation into simple components and for different industrial applications.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceCurrent Microbiology 67 (2) : 138–147 (August 2013)es_AR
dc.subjectBosqueses_AR
dc.subjectForestseng
dc.subjectBosque Primarioes_AR
dc.subjectPrimary Forestseng
dc.subjectPseudomonases_AR
dc.subjectCelulasaes_AR
dc.subjectCellulaseeng
dc.subjectActividad Enzimáticaes_AR
dc.subjectEnzyme Activityeng
dc.subject.otherBosques Nativoses_AR
dc.subject.otherArgentinaes_AR
dc.titleCharacterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forestes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.es_AR
dc.description.filFil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Sede, Silvana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentinaes_AR
dc.description.filFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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