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Abstract
A sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the milk matrix, and to be suitable for final determination by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). [ver mas...]
dc.contributor.authorMichlig, Nicolás
dc.contributor.authorRepetti, María Rosa
dc.contributor.authorChiericatti, Carolina
dc.contributor.authorGarcía, Silvia R.
dc.contributor.authorGaggiotti, Monica Del Carmen
dc.contributor.authorBasílico, Juan Carlos
dc.contributor.authorBeldoménico, Horacio Ramón
dc.date.accessioned2018-12-17T11:42:49Z
dc.date.available2018-12-17T11:42:49Z
dc.date.issued2016-09
dc.identifier.issn0009-5893
dc.identifier.issn1612-1112
dc.identifier.otherhttps://doi.org/10.1007/s10337-015-2972-1
dc.identifier.urihttps://link.springer.com/article/10.1007/s10337-015-2972-1
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4095
dc.description.abstractA sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the milk matrix, and to be suitable for final determination by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). This approach proved also to be compatible with the simultaneous extraction of pesticide residues and other contaminants (mycotoxins). Regarding AFM1, satisfactory linearity was achieved and appropriate sensitivity was maintained, using matrix-matched calibration to compensate for the heavy ion suppression. The accuracy and precision, which were determined through recovery studies, were 70–95 %, with the relative standard deviation below 15 % in all of the cases. The limit of detection (LOD, 0.002 μg L−1) and limit of quantification (0.007 μg L−1) are compatible with current worldwide regulations (maximum levels of 0.5 and 0.05 μg L−1). The procedure was applied to samples that were naturally contaminated with a range of AFM1 at LOQ–0.187 μg L−1, with comparable results to IAC clean-up, which was employed as a reference method. Therefore, AFM1 determination in raw milk by UHPLC–MS/MS detection through the present QuEChERS extraction constitutes a reliable alternative to IAC clean-up and exhibits advantages related to cost, accessibility of materials and simplicity of operation.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceChromatographia 79 (17–18) : 1091–1100 (September 2016)es_AR
dc.subjectLeche Crudaes_AR
dc.subjectRaw Milkeng
dc.subjectAflatoxinases_AR
dc.subjectAflatoxinseng
dc.subjectEspectrometría de Masases_AR
dc.subjectMass Spectrometryeng
dc.subjectMicotoxinases_AR
dc.subjectMycotoxinseng
dc.subjectPlaguicidases_AR
dc.subjectPesticideseng
dc.titleMulticlass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milkes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina.es_AR
dc.description.filFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentinaes_AR
dc.description.filFil: Chiericatti, Carolina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Microbiología; Argentinaes_AR
dc.description.filFil: García, Silvia R.. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentinaes_AR
dc.description.filFil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Basílico, Juan Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Microbiología; Argentinaes_AR
dc.description.filFil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentinaes_AR
dc.subtypecientifico


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