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Abstract
In this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could [ver mas...]
dc.contributor.authorRegiart, Daniel Matias Gaston
dc.contributor.authorFernández Baldo, Martín Alejandro
dc.contributor.authorSpotorno, Viviana Graciela
dc.contributor.authorBertolino, Franco Adrián
dc.contributor.authorRaba, Julio
dc.date.accessioned2018-10-30T12:29:49Z
dc.date.available2018-10-30T12:29:49Z
dc.date.issued2013-03-15
dc.identifier.issn0956-5663
dc.identifier.otherhttps://doi.org/10.1016/j.bios.2012.08.020
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0956566312005441
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3739
dc.description.abstractIn this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could result in human food poisoning. In order to perform the CLB detection, the SPCE was modified by gold nanoparticles (AuNPs) electrodeposition. Quantitative determination of CLB was carried out using a competitive indirect immunoassay, method based on the use of anti-CLB antibodies immobilized on magnetic micro particles. The CLB present in bovine hair samples competes immunologically with alkaline phosphatase (AP) enzyme-labeled CLB conjugate for the anti-CLB specific antibodies. Later, p-aminophenyl phosphate was converted to p-aminophenol by AP, and the electroactive product was quantified on AuNPs/SPCE at +0.1 V. The limit of detection for electrochemical method was 0.008 ng mL−1 and the intra- and inter-assay coefficients of variation were below 6%. This being a veterinary control tool very useful for rapid, sensitive and selective detection of CLB in an “in vitro” technique.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceBiosensors and Bioelectronics 41 : 211-217 (March 2013)es_AR
dc.subjectClenbuteroles_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectNanotecnologíaes_AR
dc.subjectNanotechnologyeng
dc.subjectInocuidad Alimentariaes_AR
dc.subjectFood Safetyeng
dc.subjectMedicamentos Neurotrópicoses_AR
dc.subjectNeurotropic Drugseng
dc.subject.otherInmunosensoreses_AR
dc.titleUltra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platformes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Recursos Biológicoses_AR
dc.description.filFil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentinaes_AR
dc.description.filFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentinaes_AR
dc.description.filFil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentinaes_AR
dc.description.filFil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentinaes_AR
dc.description.filFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentinaes_AR
dc.subtypecientifico


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