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Abstract
A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those [ver mas...]
dc.contributor.authorBejerman, Nicolas Esteban
dc.contributor.authorGiolitti, Fabian
dc.contributor.authorDe Breuil, Soledad
dc.contributor.authorLenardon, Sergio Luis
dc.date.accessioned2018-10-25T15:25:00Z
dc.date.available2018-10-25T15:25:00Z
dc.date.issued2013-02
dc.identifier.issn0304-8608
dc.identifier.issn1432-8798 (Online)
dc.identifier.other10.1007/s00705-012-1497-2
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3711
dc.identifier.urihttps://link.springer.com/article/10.1007/s00705-012-1497-2
dc.description.abstractA full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherSpringer; Virology Division, International Union of Microbiological Societieseng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceArchives of virology 158 (2) : 485–490. (February 2013)eng
dc.subjectHelianthus Annuuses_AR
dc.subjectVirus de las Plantases_AR
dc.subjectPlant Viruseseng
dc.subjectCloneses_AR
dc.subjectPotyviruses_AR
dc.subjectPotyviruseseng
dc.subjectInoculationeng
dc.subjectInoculaciónes_AR
dc.subject.otherGirasoles_AR
dc.subject.otherSunflowereng
dc.subject.otherSuCMoVeng
dc.subject.otherSunflower Chlorotic Mottle Viruseng
dc.subject.otherVirus del Moteado Clorótico del Girasoles_AR
dc.subject.otherInfectious Cloneeng
dc.titleDevelopment of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)eng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentinaes_AR
dc.subtypecientifico


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