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A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those
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dc.contributor.author | Bejerman, Nicolas Esteban | |
dc.contributor.author | Giolitti, Fabian | |
dc.contributor.author | De Breuil, Soledad | |
dc.contributor.author | Lenardon, Sergio Luis | |
dc.date.accessioned | 2018-10-25T15:25:00Z | |
dc.date.available | 2018-10-25T15:25:00Z | |
dc.date.issued | 2013-02 | |
dc.identifier.issn | 0304-8608 | |
dc.identifier.issn | 1432-8798 (Online) | |
dc.identifier.other | 10.1007/s00705-012-1497-2 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/3711 | |
dc.identifier.uri | https://link.springer.com/article/10.1007/s00705-012-1497-2 | |
dc.description.abstract | A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | Springer; Virology Division, International Union of Microbiological Societies | eng |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Archives of virology 158 (2) : 485–490. (February 2013) | eng |
dc.subject | Helianthus Annuus | es_AR |
dc.subject | Virus de las Plantas | es_AR |
dc.subject | Plant Viruses | eng |
dc.subject | Clones | es_AR |
dc.subject | Potyvirus | es_AR |
dc.subject | Potyviruses | eng |
dc.subject | Inoculation | eng |
dc.subject | Inoculación | es_AR |
dc.subject.other | Girasol | es_AR |
dc.subject.other | Sunflower | eng |
dc.subject.other | SuCMoV | eng |
dc.subject.other | Sunflower Chlorotic Mottle Virus | eng |
dc.subject.other | Virus del Moteado Clorótico del Girasol | es_AR |
dc.subject.other | Infectious Clone | eng |
dc.title | Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV) | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | Instituto de Patología Vegetal | es_AR |
dc.description.fil | Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina | es_AR |
dc.subtype | cientifico |
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