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Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
Abstract
A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those
[ver mas...]
A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.
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![Thumbnail](/xmlui/bitstream/handle/20.500.12123/3711/cover_archives%20of%20virology_2013.jpg?sequence=3&isAllowed=y)
Fuente
Archives of virology 158 (2) : 485–490. (February 2013)
Date
2013-02
Editorial
Springer; Virology Division, International Union of Microbiological Societies
ISSN
0304-8608
1432-8798 (Online)
1432-8798 (Online)
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Derechos de acceso
Restringido
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