Show simple item record

resumen

Abstract
Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this [ver mas...]
dc.contributor.authorTortone, Claudia Andrea
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorGioffre, Andrea
dc.contributor.authorOriani, Delia Susana
dc.date.accessioned2018-10-18T12:48:22Z
dc.date.available2018-10-18T12:48:22Z
dc.date.issued2018-03
dc.identifier.issn2212-5531
dc.identifier.otherDOI: 10.4103/ijmy.ijmy_192_17
dc.identifier.urihttp://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=53;epage=60;aulast=Tortone
dc.identifier.urihttp://hdl.handle.net/20.500.12123/3625
dc.description.abstractBackground: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65-PRA (PCR-Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp 65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65-PRA, partial sequencing of 16S rRNA and sequencing of hsp 65 genes. Results: Phenotypic evaluation and hsp65-PRA were concordant with 23 (41.1%) isolates. Also, the PRA was concordant with 16 (28.6%) and 27 (48.2%) isolates, with the partial sequencing of 16S rRNA and sequencing of hsp 65 genes, respectively. It is considered that the 19.6% (n = 11) could not be identified. Conclusion: Identification of NTM environmental isolates to the species level, especially when they are pigmented and fast-growing, both the analysis of the restriction patterns obtained by PRA and the sequencing of the 16S rRNA and hsp 65 genes are insufficient by themselves. Although they are demanding and time-consuming, biochemical tests are very useful to support data obtained by molecular methods.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWolters Kluwer - Medknowes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceInternational Journal of Mycobacteriology 7 (1): 53-60 (Marzo 2018)es_AR
dc.subjectEnzymatic Analysiseng
dc.subjectAnálisis Enzimáticoes_AR
dc.subjectWaterborne Diseaseseng
dc.subjectEnfermedades Transmitidas por Aguaes_AR
dc.subject.otherNontuberculous Mycobacteriaeng
dc.subject.otherMicobacterias no Tuberculosases_AR
dc.subject.otherMétodos Fenotípicoses_AR
dc.subject.otherPhenotypic Methodseng
dc.subject.otherFuentes de Aguaes_AR
dc.subject.otherWater Sourceseng
dc.titleUtilization of molecular and conventional methods for the identification of nontuberculous mycobacteria isolated from different water sourceses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.subtypecientfico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record