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Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
Luño, Victoria; Gil, María Lydia; Olaciregui, Maite; Jerez, Rodrigo A.; De Blas, José Ignacio; Hozbor, Federico Andres (Wiley, 2015-11)In this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected ... -
Biotécnicas reproductivas en la especie porcina: pasado, presente y futuro
Marinone, Ana Ines; Kaiser, German Gustavo; Hozbor, Federico Andres; Mucci, Nicolas Crescencio (2018-08)Argentina posee condiciones agroecológicas y sanitarias ideales para la producción de cerdos. Hasta 1990, la producción porcina en Argentina era realizada como actividad secundaria dentro de la explotación agropecuaria. ... -
Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
Manes, Jorgelina; Fiorentino, María Andrea; Kaiser, German Gustavo; Hozbor, Federico Andres; Alberio, Ricardo; Sánchez, Esteban O.; Paolicchi, Fernando (Elsevier, 2010-11)The objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices ... -
Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality
Carro, María De Las Mercede; Peñalva, Daniel; Antollini, Silvia; Hozbor, Federico Andres; Buschiazzo, Jorgelina (Elsevier, 2020-09)Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs. We evaluated the extent of cholesterol and desmosterol incorporation ... -
Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation Improves in vitro and in vivo Fertility
Carro, María de las Mercedes; Ramírez Vasquez, Rafael R.; Peñalva, Daniel A.; Buschiazzo, Jorgelina; Hozbor, Federico Andres (Frontiers Media, 2021-06)Pregnancy rates in ewes are markedly low after cervical insemination with frozen-thawed sperm. Sensitivity of ram sperm to freeze-thawing is related to the lipid composition of the membrane, particularly to its low sterol ... -
Effect of Seminal Plasma on Post‐Thaw Quality and Functionality of Corriedale Ram Sperm Obtained by Electroejaculation and Artificial Vagina
Ledesma, Alba; Manes, Jorgelina; Rios, Glenda Laura; Aller Atucha, Juan Florencio; Cesari, Andreina; Alberio, Ricardo; Hozbor, Federico Andres (Wiley, 2015-06)We have already shown that seminal collection method affects seminal plasma composition and sperm quality in Corriedale rams. In this study, we evaluated the effect of seminal plasma collected by electroejaculation or ... -
Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams
Ledesma, Alba; Manes, Jorgelina; Cesari, Andreina; Alberio, Ricardo; Hozbor, Federico Andres (Wiley, 2014-04)This study was conducted to evaluate the effect of seminal collection method (artificial vagina or electroejaculation) on the protein composition of seminal plasma and sperm quality parameters in Corriedale rams. To address ... -
Heterologous recombinant protein with decapacitating activity prevents and reverts cryodamage in ram sperm: An emerging biotechnological tool for cryobiology
Zalazar, Lucía; Ledesma, Alba Verónica; Hozbor, Federico Andres; Cesari, Andreína (Elsevier, 2016-01)During the last decades fundamental and applied aspects of mammalian ram sperm cryopreservation have been increasingly explored by scientists and biotechnologists. Many works report modifications in the composition of the ... -
Improve intra‐uterine insemination in rabbits using ultra‐high temperature skim milk as extender to keep semen at room temperature
Hozbor, Federico Andres; Ledesma, Alba; Manes, Jorgelina; Rios, Glenda Laura; Kaiser, German Gustavo; Cano, Adriana Virginia; Luciano, Cecilia Ines; Alberio, Ricardo (Wiley, 2016-03)Two experiments were carried out to examine in vitro quality and in vivo fertility of rabbit semen diluted in ultra‐high temperature (UHT) skim milk. In the first experiment, pooled ejaculates of 10 adult rabbits were ... -
PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender
Carro, María De Las Mercedes; Luquez, J.M.; Peñalva, D.A.; Buschiazzo, Jorgelina; Hozbor, Federico Andres; Furland, N.E. (Elsevier, 2022-04)Cryopreservation is known to affect spermatozoa structure and function. Ram sperm are among the most highly sensitive mammalian gametes to freezing, due to their lipid composition, which limit their efficiency in artificial ... -
Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
Ledesma, Alba; Fernández Alegre, Estela; Cano, Adriana Virginia; Hozbor, Federico Andres; Martínez Pastor, Felipe; Cesari, Andreina (Elsevier, 2016-10)This study was conducted to evaluate the effects of interacting seminal plasma proteins (iSPP) obtained by AV or EE on frozen-thawed ram sperm in order to test the hypothesis whether this fraction could be sufficient to ... -
Vaginal histological changes after using intravaginal sponges for oestrous synchronization in anoestrous ewes
Manes, Jorgelina; Campero, Carlos Manuel; Hozbor, Federico Andres; Alberio, Ricardo; Ungerfeld, Rodolfo (Wiley, 2015-04)To characterize the histological and cytological vaginal changes generated by the use of intravaginal sponge (IS) applied in oestrous synchronization treatments in ewes during mid‐non‐breeding season. Thirty‐five multiparous ...