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Detection of Mycobacterium bovis in Bovine and Bubaline tissues using Nested-PCR for TbD1
(PLOS, 2014-03)In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. ... -
Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
(Mary Ann Liebert Inc., 2012-02)Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we ... -
Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
(2017-12)Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have ... -
Development of highly sensitive one step-PCR tests for improved detection of B. bigemina and B. bovis
(Elsevier, 2021-08)Bovine babesiosis caused by Babesia bigemina and B. bovis is an economically relevant tick-borne disease distributed over tropical and subtropical world regions. Animals that recover from the clinical disease can remain ... -
Diagnóstico de Entamoeba polecki y su potencial impacto en las condiciones sanitarias de la producción porcina
(Ediciones INTA, 2019-12)En Argentina, la producción porcina constituye una actividad en constante aumento, en particular, para la pequeña agricultura familiar. No obstante, este tipo de producción posee ciertas limitaciones (estructurales y ... -
L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus
(2019-11)Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive ... -
Preparation of a working seed lot of BCG and quality control by PCR genotyping
(Asociación Argentina de Microbiología, 2010-04)The bacillus Calmette-Guérin (BCG) was obtained in 1920 after successive passages leading to the attenuation of a Mycobacterium bovis strain. For the following 40 years, BCG had been replicated, resulting in substrains ... -
Tuberculosis in swine co-infected with Mycobacterium avium subsp. hominissuis and Mycobacterium bovis in a cluster from Argentina
(Cambridge University Press, 2015-04)In Argentina little is known about the epidemiology of tuberculosis (TB) infection in swine. We characterized the epidemiological dynamics of Mycobacterium avium complex (MAC) infection in a swine population of Argentina ... -
Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
(Sage Publications, 2005-05)The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and ... -
Validation of reference genes for quantitative PCR in Johnsongrass (Sorghum halepense L.) under glyphosate stress
(MDPI, 2021-08)Weeds are one of the main causes of the decrease in crop yields, with Johnsongrass (Sorghum halepense L.) being one of the most significant. Weeds can be controlled by herbicides, but some have developed resistance. ...