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Mostrando ítems 1-7 de 7

    • Efficient CRISPR/Cas9 genome editing in alfalfa using a public germplasm 

      Bottero, Ana Emilia; Massa, Gabriela Alejandra; Gonzalez, Matías Nicolás; Stritzler, Margarita; Tajima, Hiromi; Gomez, Maria Cristina; Frare, Romina Alejandra; Feingold, Sergio Enrique; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Frontiers Media, 2021-07)
      Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source of vegetable proteins in meat and milk production systems in temperate regions. Alfalfa cultivation also adds ...

    • Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia 

      Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Galindo Sotomonte, Luisa Fernanda; McCormick, Wayne; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2022-02)
      We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present ...

    • Generation of a multi‑herbicide‑tolerant alfalfa by using base editing 

      Bottero, Ana Emilia; Gomez, Maria Cristina; Stritzler, Margarita; Tajima, Hiromi; Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-02)
      We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important ...

    • Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing 

      Stritzler, Margarita; Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Gomez, Maria Cristina; Frare, Romina Alejandra; Puebla, Andrea Fabiana; Tajima, Hiromi; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)
      The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ...

    • Spontaneous Mutations in the Nitrate Reductase Gene napC Drive the Emergence of Eco-friendly Low-N2O-Emitting Alfalfa Rhizobia in Regions with Different Climates 

      Brambilla, Silvina Maricel; Soto, Gabriela; Odorizzi, Ariel; Arolfo, Valeria; McCormick, Wayne; Primo, Emiliano; Giordano, Walter; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Springer, 2020)
      We have recently shown that commercial alfalfa inoculants (e.g., Sinorhizobium meliloti B399), which are closely related to the denitrifier model strain Sinorhizobium meliloti 1021, have conserved nitrate, nitrite, and ...

    • Subfunctionalization probably drives the emergence of plant growth‑promoting genes 

      Frare, Romina Alejandra; Mozzicafreddo, Matteo; Gomez, Maria Cristina; Fussoni, Nerina Belen; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2022-07)
      The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution ...

    • The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa 

      Gomez, Maria Cristina; Jozefkowicz, Cintia; Mozzicafreddo, Matteo; Odorizzi, Ariel; Arolfo, Valeria; Basigalup, Daniel Horacio; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-12)
      The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial ...