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Mostrando ítems 21-40 de 45
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Optimizing ddRADseq in non-model species: a case study in Eucalyptus dunnii maiden
Aguirre, Natalia Cristina; Filippi, Carla Valeria; Zaina, Giusi; Rivas, Juan Gabriel; Acuña, Cintia Vanesa; Villalba, Pamela Victoria; Garcia, Martin Nahuel; Gonzalez, Sergio; Rivarola, Maximo Lisandro; Martinez, Maria Carolina; Puebla, Andrea Fabiana; Morgante, Michele; Hopp, Horacio Esteban; Paniego, Norma Beatriz; Marcucci Poltri, Susana Noemi (MDPI, 2019-08)Restriction site-associated DNA sequencing (RADseq) and its derived protocols, such as double digest RADseq (ddRADseq), offer a flexible and highly cost-effective strategy for efficient plant genome sampling. This has ... -
The overexpression of antifungal genes enhances resistance to rhizoctonia solani in transgenic potato plants without affecting arbuscular mycorrhizal symbiosis
Fernandez Bidondo, Laura; Almasia, Natalia Ines; Bazzini, Ariel Alejandro; Colombo, Roxana; Hopp, Horacio Esteban; Vazquez Rovere, Cecilia; Godeas, Alicia (Elsevier, 2019-10)The biological control of fungal diseases through the use of genetically modified (GM) plants could decrease the input of chemical pesticides. To overcome possible losses in potato (Solanum tuberosum) yield because of ... -
Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
Almasia, Natalia Ines; Bazzini, Ariel Alejandro; Hopp, Horacio Esteban; Vazquez Rovere, Cecilia (Wiley-Blackwell, 2008-05)Snakin‐1 (SN1), a cysteine‐rich peptide with broad‐spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned ... -
P0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistance
Agrofoglio, Yamila Carla; Delfosse, Veronica Cecilia; Casse, Maria Florencia; Hopp, Horacio Esteban; Bonacic Kresic, Iván; Ziegler-Graff, Véronique; Distefano, Ana Julia (Wiley, 2019-08)Cotton blue disease (CBD) is the most important disease present in cotton crops in South America and cotton leafroll dwarf virus (CLRDV) is the causal agent. The disease has been controlled by sowing cotton varieties ... -
Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
Darqui, Flavia Soledad; Radonic, Laura Mabel; Beracochea, Valeria Cecilia; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (Frontiers Publishing, 2021-11)The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated ... -
Phenotyping sunflower genetic resources for Sclerotinia head rot response: assessing variability for disease resistance breeding
Filippi, Carla Valeria; Zubrzycki, Jeremias Enrique; Di Rienzo, Julio A.; Quiroz, Facundo Jose; Fusari, Corina Mariana; Alvarez, Daniel; Maringolo, Carla Andrea; Cordes, Diego Darío; Escande, Alberto; Hopp, Horacio Esteban; Heinz, Ruth Amelia; Lia, Veronica Viviana; Paniego, Norma Beatriz (The American Phytopathological Society, 2017-11)Sclerotinia head rot (SHR) is one of the most serious constraints to sunflower (Helianthus annuus L. var. macrocarpus) production worldwide. Here, we evaluated the response to SHR in a sunflower inbred panel from a large ... -
Plastome genomics in South American maize landraces: chloroplast lineages parallel the geographic structuring of nuclear gene pools
Lopez, Mariana Gabriela; Fass, Monica Irina; Rivas, Juan Gabriel; Carbonell-Caballero, José; Vera, Pablo Alfredo; Puebla, Andrea Fabiana; Defacio, Raquel Alicia; Dopazo, Joaquín; Paniego, Norma Beatriz; Hopp, Horacio Esteban; Lia, Verónica Viviana (Oxford University Press, 2021-03)Background and Aims: The number of plastome sequences has increased exponentially during the last decade. However, there is still little knowledge of the levels and distribution of intraspecific variation. The aims of this ... -
Population genetics structure of glyphosate‐resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance
Fernandez, Luis German; De Haro, Luis Alejandro; Distefano, Ana Julia; Martinez, Maria Carolina; Lia, Veronica Viviana; Papa, Juan Carlos; Olea, Ignacio; Tosto, Daniela Sandra; Hopp, Horacio Esteban (Wiley, 2013-09)Single sequence repeats (SSR) developed for Sorghum bicolor were used to characterize the genetic distance of 46 different Sorghum halepense (Johnsongrass) accessions from Argentina some of which have evolved toward ... -
Population structure and genetic diversity characterization of a sunflower association mapping population using SSR and SNP markers
Filippi, Carla Valeria; Aguirre, Natalia Cristina; Rivas, Juan Gabriel; Zubrzycki, Jeremias Enrique; Puebla, Andrea Fabiana; Cordes, Diego Darío; Moreno, Maria Valeria; Fusari, Corina Mariana; Alvarez, Daniel; Heinz, Ruth Amelia; Hopp, Horacio Esteban; Paniego, Norma Beatriz; Lia, Veronica Viviana (BMC, 2015-02)Background: Argentina has a long tradition of sunflower breeding, and its germplasm is a valuable genetic resource worldwide. However, knowledge of the genetic constitution and variability levels of the Argentinean germplasm ... -
Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants
Darqui, Flavia Soledad; Radonic, Laura Mabel; Trotz, Paulina M.; Lopez, Nilda Ester; Vazquez Rovere, Cecilia; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (Elsevier, 2018-10)Snakin-1 is a cysteine-rich antimicrobial peptide (AMP) isolated from potato tubers, with broad-spectrum activity. It belongs to the Snakin/GASA family, whose members have been studied because of their diverse roles ... -
Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition
Nahirñak, Vanesa; Almasia, Natalia Ines; Fernandez, Paula Virginia; Hopp, Horacio Esteban; Estevez, Jose Manuel; Carrari, Fernando; Vazquez Rovere, Cecilia (American Society of Plant Biologists, 2012-01)Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a ... -
Potato snakin‑1: an antimicrobial player of the trade‑off between host defense and development
Almasia, Natalia Ines; Nahirñak, Vanesa; Hopp, Horacio Esteban; Vazquez Rovere, Cecilia (Springer, 2020-07)Snakin-1 (SN1) from potato is a cysteine-rich antimicrobial peptide with high evolutionary conservation. It has 63 amino acid residues, 12 of which are cysteines capable of forming six disulfide bonds. SN1 localizes in the ... -
Sequence analysis of genome segments S5 and S10 of Mal de Río Cuarto virus (Fijivirus, Reoviridae)
Distefano, Ana Julia; Hopp, Horacio Esteban; Del Vas, Mariana (Springer, 2005-06)Mal de Río Cuarto virus (MRCV) was recently described as a new species of the genus Fijivirus, family Reoviridae. The nucleotide sequence of two MRCV genome segments was determined. MRCV S5 and S10 were predicted to encode ... -
Simplified methodology for large scale isolation of homozygous transgenic lines of lettuce
Darqui, Flavia Soledad; Radonic, Laura Mabel; Lopez, Nilda Ester; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (2018-01)Background: Lettuce is a globally important leafy vegetable and a model plant for biotechnology due to its adaptability to tissue culture and stable genetic transformation. Lettuce is also crucial for functional genomics ... -
Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
Nahirñak, Vanesa; Rivarola, Maximo Lisandro; Almasia, Natalia Ines; Barrios Baron, María Pilar; Hopp, Horacio Esteban; Vile, Denis; Paniego, Norma Beatriz; Vazquez Rovere, Cecilia (PLOS, 2019-03-25)Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action ... -
Snakin/GASA proteins : involvement in hormone crosstalk and redox homeostasis
Nahirñak, Vanesa; Almasia, Natalia Ines; Hopp, Horacio Esteban; Vazquez Rovere, Cecilia (Taylor & Francis, 2012)Snakin/GASA proteins are widely distributed among plant species. They are expressed in different plant organs with high tissue and temporal specificity, and their subcellular localization varies among the different members. ... -
State of the art of genetic engineering in potato: from the first report to its future potential
Nahirñak, Vanesa; Almasia, Natalia Ines; Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Decima Oneto, Cecilia Andrea; Feingold, Sergio Enrique; Hopp, Horacio Esteban; Vazquez Rovere, Cecilia (Frontiers Media, 2022-01)Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, ... -
Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana
Beracochea, Valeria Cecilia; Almasia, Natalia Ines; Peluffo, Lucila; Nahirñak, Vanesa; Hopp, Horacio Esteban; Paniego, Norma Beatriz; Heinz, Ruth Amelia; Vazquez Rovere, Cecilia; Lia, Veronica Viviana (Springer, 2015-10)Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant ... -
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
Darqui, Flavia Soledad; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (Crimson Publishers, 2020-08)Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been ... -
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing
Delfosse, Veronica Cecilia; Agrofoglio, Yamila Carla; Casse, Maria Florencia; Bonacic Kresic, Iván; Hopp, Horacio Esteban; Ziegler-Graff, Véronique; Distefano, Ana Julia (2014-02)Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded ...