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Mostrando ítems 1-10 de 10
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A thermostable GH8 endoglucanase of Enterobacter sp. R1 is suitable for β- glucan deconstruction
Ontañon, Ornella Mailen; Ghio, Silvina; Marrero Diaz De Villegas, Rubén; Garrido, Mercedes Maria; Talia, Paola Mónica; Fehér, Csaba; Campos, Eleonora (Elsevier, 2019-11)Glycoside hydrolase family 8 (GH8) includes endoglucanases, lichenases, chitosanases and xylanases, which are essential for polysaccharides breakdown. In this work, we studied a thermally stable GH8 from the cellulose ... -
Draft Genome Sequence of Cellulolytic and Xylanolytic Cellulomonas sp. Strain B6 Isolated from Subtropical Forest Soil
Piccinni, Florencia Elizabeth; Murua, Yanina; Ghio, Silvina; Talia, Paola Mónica; Rivarola, Maximo Lisandro; Campos, Eleonora (2016-08)The genome information will be useful for studies of microbial enzymes for industrial application in lignocellulosic biomass utilization. -
EcXyl43 β-xylosidase: molecular modeling, activity on natural and artificial substrates, and synergism with endoxylanases for lignocellulose deconstruction
Ontañon, Ornella Mailén; Ghio, Silvina; Marrero Diaz De Vill, Rubén; Piccinni, Florencia Elizabeth; Talia, Paola Mónica; Cerutti, Maria Laura; Campos, Eleonora (Springer, 2018-08)Biomass hydrolysis constitutes a bottleneck for the biotransformation of lignocellulosic residues into bioethanol and high-value products. The efficient deconstruction of polysaccharides to fermentable sugars requires ... -
Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
Romero Victorica, Matias; Soria, Marcelo Abel; Batista-García, Ramón Alberto; Ceja-Navarro, Javier A.; Vikram, Surendra; Ortiz, Maximiliano; Ontañon, Ornella; Ghio, Silvina; Martínez-Ávila, Liliana; Quintero García, Omar Jasiel; Etcheverry, Clara; Campos, Eleonora; Cowan, Donald; Arneodo Larochette, Joel Demian; Talia, Paola Mónica (Springer Nature, 2020-03-02)In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different ... -
Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
Ontañon, Ornella Mailen; Bedő, Soma; Ghio, Silvina; Garrido, Mercedes Maria; Topalian, Juliana; Jahola, Dóra; Fehér, Anikó; Valacco, Maria Pia; Campos, Eleonora; Fehér, Csaba (Springer, 2021-05)One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still ... -
Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
Coniglio, Romina O.; Fonseca, María I.; Diaz, Gabriela V.; Ontañon, Ornella Mailén; Ghio, Silvina; Campos, Eleonora; Zapata, Pedro Darío (Taylor & Francis, 2019-04)The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing ... -
Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil
Ghio, Silvina; Sauka, Diego Hernan; Ferrari, Alejandro E.; Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Campos, Eleonora (Microbiology Society, 2019-12)A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina. Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium. Its ... -
Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Ghio, Silvina; Sauka, Diego Hernan; Talia, Paola Mónica; Rivarola, Maximo Lisandro; Valacco, María Pía; Campos, Eleonora (Wiley; Society for Applied Microbiology, 2018-12)Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual ... -
Structural and molecular dynamics investigations of ligand stabilization via secondary binding site interactions in Paenibacillus xylanivorans GH11 xylanase
Briganti, Lorenzo; Capetti, Caio; Pellegrini, Vanessa O. A.; Ghio, Silvina; Campos, Eleonora; Nascimento, Alessandro S.; Polikarpov, Igor (Elsevier, 2021-03)Glycoside hydrolases (GHs) are essential for plant biomass deconstruction. GH11 family consist of endo-β-1,4-xylanases which hydrolyze xylan, the second most abundant cell wall biopolymer after cellulose, into small ... -
Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversio
Ghio, Silvina; Bradanini, María B.; Garrido, Mercedes Maria; Ontañon, Ornella Mailen; Piccinni, Florencia Elizabeth; Marrero Diaz De Vill, Rubén; Talia, Paola Mónica; Campos, Eleonora (Elsevier, 2020-12)In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase ...