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Abstract
Objectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage‐like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. [ver mas...]
dc.contributor.authorBertona, Daiana
dc.contributor.authorPujato, Nazarena
dc.contributor.authorBontempi, Iván
dc.contributor.authorGonzález, Verónica Doris Guadalupe
dc.contributor.authorCabrera, Gabriel Gustavo
dc.contributor.authorGugliotta, Luis Marcelino
dc.contributor.authorHozbor, Daniela Flavia
dc.contributor.authorNicastro, Alcides
dc.contributor.authorCalvinho, Luis Fernando
dc.contributor.authorMarcipar, Iván Sergio
dc.date.accessioned2018-07-18T14:14:26Z
dc.date.available2018-07-18T14:14:26Z
dc.date.issued2017-10
dc.identifier.issn0022-3573
dc.identifier.issn2042-7158
dc.identifier.otherhttps://doi.org/10.1111/jphp.12768
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/abs/10.1111/jphp.12768
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2813
dc.description.abstractObjectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage‐like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX™ performance. Particle size and adjuvant performance were conserved during the 6‐month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans‐sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceJournal of Pharmacy and Pharmacology 69 (10) : 1293-1303 (October 2017)es_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectTrypanosoma cruzies_AR
dc.subjectCoadyuvanteses_AR
dc.subjectAdjuvantseng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectImmune Responseeng
dc.titleDevelopment and assessment of a new cage‐like particle adjuvantes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Bertona, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentinaes_AR
dc.description.filFil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentinaes_AR
dc.description.filFil: Bontempi, Iván. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentinaes_AR
dc.description.filFil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral; Argentinaes_AR
dc.description.filFil: Cabrera, Gabriel Gustavo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Gugliotta, Luis Marcelino. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentinaes_AR
dc.description.filFil: Hozbor, Daniela Flavia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Laboratorio VacSal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Nicastro, Alcides. LIPOMIZE SRL Santa Fe; Argentinaes_AR
dc.description.filFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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