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Resumen
Staphylococcus aureus produces capsular polysaccharides (CPs) both in vivo and under defined culture conditions being serotypes 5 and 8 the most prevalent. S. aureus isolates that fail to produce CP5 or CP8 are defined as non-typeable (NT). Loss of capsule expression, however, may lead to S. aureus persistence in a chronically infected host. The prevalence of NT strains of S. aureus isolated from bovine mastitis varies according to the geographic origin [ver mas...]
dc.contributor.authorAmbroggio, Maria Belen
dc.contributor.authorPerrig, Melina Soledad
dc.contributor.authorCamussone, Cecilia
dc.contributor.authorPujato, Nazarena
dc.contributor.authorBertón, Alicia
dc.contributor.authorGianneechini, Edgardo
dc.contributor.authorAlvarez, Silvia
dc.contributor.authorMarcipar, Ivan Sergio
dc.contributor.authorCalvinho, Luis Fernando
dc.contributor.authorBarbagelata, Maria Sol
dc.coverage.spatialArgentina (nation)
dc.coverage.spatialChile (nation)
dc.coverage.spatialUruguay (nation)
dc.dateinfo:eu-repo/date/embargoEnd/2019-08-30
dc.date.accessioned2018-07-18T12:08:43Z
dc.date.available2018-07-18T12:08:43Z
dc.date.issued2018-08
dc.identifier.issn1234-1983
dc.identifier.issn2190-3883
dc.identifier.otherhttps://doi.org/10.1007/s13353-018-0443-8
dc.identifier.urihttps://link.springer.com/article/10.1007/s13353-018-0443-8
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2811
dc.description.abstractStaphylococcus aureus produces capsular polysaccharides (CPs) both in vivo and under defined culture conditions being serotypes 5 and 8 the most prevalent. S. aureus isolates that fail to produce CP5 or CP8 are defined as non-typeable (NT). Loss of capsule expression, however, may lead to S. aureus persistence in a chronically infected host. The prevalence of NT strains of S. aureus isolated from bovine mastitis varies according to the geographic origin of the strain. The aims of this work were to detect phenotypically and genotypically the capsular profile of 144 S. aureus isolated from bovine mastitis in Argentina, Chile, and Uruguay and explore the factors that are considered to be associated with capsule expression as presence of IS257, IScap, and agr typing of non-related collection. The detection of the IS257, IScap, cap genes, and agr typing was performed using PCR. The detection and quantification of capsular polysaccharide production were performed by ELISA assays. We found that 96% of the S. aureus isolates investigated carried cap5(8) genes but over 75% of strains do not express capsule in the three countries studied. However, only 6 isolates from Argentina carried the IScap element that totally suppressed the expression of the capsule, suggesting that other factors could influence on CP expression. Moreover, the agrI/NT association was statistically significant suggesting that this profile is a phenomenon observed not only in other parts of the world but also in our region.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_AR
dc.sourceJournal of Applied Genetics 59 (3) : 357–363 (August 2018)es_AR
dc.subjectGanado de Lechees_AR
dc.subjectDairy Cattleeng
dc.subjectStaphylococcus aureuses_AR
dc.subjectMastítis Bovinaes_AR
dc.subjectBovine Mastitiseng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectPolisacáridoses_AR
dc.subjectPolysaccharideseng
dc.subject.otherArgentinaes_AR
dc.subject.otherChilees_AR
dc.subject.otherUruguayes_AR
dc.titleSurvey of potential factors involved in the low frequency of CP5 and CP8 expression in Staphylococcus aureus isolates from mastitis of dairy cattle from Argentina, Chile, and Uruguayes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Ambroggio, Maria Belen. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentinaes_AR
dc.description.filFil: Perrig, Melina Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentinaes_AR
dc.description.filFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentinaes_AR
dc.description.filFil: Bertón, Alicia. Lactodiagnóstico Sur Uruguay. Colonia Cosmopolita ; Uruguayes_AR
dc.description.filFil: Gianneechini, Edgardo. Laboratory Veterinary Direction “Miguel C. Rubino”; Uruguayes_AR
dc.description.filFil: Alvarez, Silvia. Udder Health; Chilees_AR
dc.description.filFil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Barbagelata, Maria Sol. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentinaes_AR
dc.subtypecientifico


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