Facebook
Twitter
YouTube
Instagram
    • español
    • English
  • Contacto
  • English 
    • español
    • English
  • Login

Inta Digital

Repositorio InstitucionalBiblioteca Digital
AboutAuthorsTitlesSubjectsCollectionsCommunities☰
JavaScript is disabled for your browser. Some features of this site may not work without it.
View Item 
    xmlui.general.dspace_homeCentros Regionales y EEAsCentro Regional CórdobaEEA ManfrediArtículos científicosxmlui.ArtifactBrowser.ItemViewer.trail
  • DSpace Home
  • Centros Regionales y EEAs
  • Centro Regional Córdoba
  • EEA Manfredi
  • Artículos científicos
  • View Item

A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies

Abstract
The development of high-yielding crops with drought tolerance is necessary to increase food, feed, fiber and fuel production. Methods that create similar environmental conditions for a large number of genotypes are essential to investigate plant responses to drought in gene discovery studies. Modern facilities that control water availability for each plant remain cost-prohibited to some sections of the research community. We present an alternative [ver mas...]
The development of high-yielding crops with drought tolerance is necessary to increase food, feed, fiber and fuel production. Methods that create similar environmental conditions for a large number of genotypes are essential to investigate plant responses to drought in gene discovery studies. Modern facilities that control water availability for each plant remain cost-prohibited to some sections of the research community. We present an alternative cost-effective automated irrigation system scalable for a high-throughput and controlled dry-down treatment of plants. This system was tested in sorghum using two experiments. First, four genotypes were subjected to ten days of dry-down to achieve three final Volumetric Water Content (VWC) levels: drought (0.10 and 0.20 m3 m-3) and control (0.30 m3 m-3). The final average VWC was 0.11, 0.22, and 0.31 m3 m-3, respectively, and significant differences in biomass accumulation were observed between control and drought treatments. Second, 42 diverse sorghum genotypes were subjected to a seven-day dry-down treatment for a final drought stress of 0.15 m3 m-3 VWC. The final average VWC was 0.17 m3 m-3, and plants presented significant differences in photosynthetic rate during the drought period. These results demonstrate that cost-effective automation systems can successfully control substrate water content for each plant, to accurately compare their phenotypic responses to drought, and be scaled up for high-throughput phenotyping studies. [Cerrar]
Thumbnail
Author
Ortiz, Diego;   Litvin, Alexander G.;   Salas Fernandez, Marìa G.;  
Fuente
PLoS ONE 13 (6) : e0198546 (2018)
Date
2018-06-05
ISSN
1932-6203
URI
http://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0198546
http://hdl.handle.net/20.500.12123/2697
DOI
https://doi.org/10.1371/journal.pone.0198546
Documentos Relacionados
Formato
pdf
Tipo de documento
artículo
Derechos de acceso
Abierto
Descargar
Compartir
  • Compartir
    Facebook Email Twitter Mendeley
Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Metadata
Show full item record