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Abstract
An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of [ver mas...]
dc.contributor.authorMartínez, Diana
dc.contributor.authorThompson, Carolina Soledad
dc.contributor.authorDraghi, Maria Graciela
dc.contributor.authorCanavesio, Vilma
dc.contributor.authorJacobo, Roberto
dc.contributor.authorZimmer, Patricia Andrea
dc.contributor.authorElena, Sebastián
dc.contributor.authorNicola, Ana M.
dc.contributor.authorTorioni, Susana Marta
dc.date.accessioned2018-06-28T13:59:03Z
dc.date.available2018-06-28T13:59:03Z
dc.date.issued2014-09-17
dc.identifier.issn0378-1135
dc.identifier.issn1873-2542
dc.identifier.otherhttps://doi.org/10.1016/j.vetmic.2014.07.011
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0378113514003344?via%3Dihub
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2688
dc.description.abstractAn isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus–B. melitensis–B. ovis–B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis. Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498 bp) and B. melitensis (731 bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVeterinary Microbiology 173 (1–2) : 172-176 (September 2014)es_AR
dc.subjectBúfalo de Aguaes_AR
dc.subjectWater Buffaloeseng
dc.subjectBrucella Abortuses_AR
dc.subjectGenotiposes_AR
dc.subjectGenotypeseng
dc.subjectFenotiposes_AR
dc.subjectPhenotypeseng
dc.subjectBrucelosises_AR
dc.subjectBrucellosiseng
dc.subject.otherAmérica Latinaes_AR
dc.titlePheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americases_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Martinez, Diana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Draghi, Maria Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentinaes_AR
dc.description.filFil: Canavesio, Vilma. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Jacobo, Roberto. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Zimmer, Patricia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentinaes_AR
dc.description.filFil: Elena, Sebastián. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; Argentinaes_AR
dc.description.filFil: Nicola, Ana M. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; Argentinaes_AR
dc.description.filFil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.subtypecientifico


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