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Mostrando ítems 1-16 de 16
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A Trypanosoma species detected in Rhipicephalus (Boophilus) microplus ticks from Argentina
Morel, Nicolas; Thompson, Carolina Soledad; Rossner, Maria Victoria; Mangold, Atilio Jose; Nava, Santiago (Elsevier, 2020-09)Specimens of a Trypanosoma sp. were found in a haemolymph sample of Rhipicephalus microplus from Argentina. Polymerase chain reaction (PCR) was done targeting the SSU rRNA gene of Trypanosoma spp. and a fragment of 2300 ... -
Babesia bovis biological clones and the inter-strain allelic diversity of the Bv80 gene support subpopulation selection as a mechanism involved in the attenuation of two virulent isolates
Baravalle, María Eugenia; Thompson, Carolina Soledad; Valentini, Beatriz Susana; Ferreira, Mariano B.; Torioni, Susana Marta; Florin-Christensen, Mónica; Echaide, Ignacio Eduardo (Elsevier, 2012-12-21)The virulence phenotype of Babesia bovis subpopulations was evaluated using biological clones derived from the high-virulence BboS2P and the low-virulence BboR1A strain and two original virulent isolates, BboL15 and BboL17, ... -
Biochemical characterization of thioredoxin reductase from Babesia bovis
Regner, Erika Lucía; Thompson, Carolina Soledad; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian; Arias, Diego Gustavo (2014-04)This paper addresses the identification, cloning, expression, purification and functional characterization of thioredoxin reductase from Babesia bovis, the etiological agent of babesiosis. The work deals with in vitro ... -
Development and evaluation of a double-antigen sandwich ELISA to identify Anaplasma marginale–infected and A. centrale–vaccinated cattle
Sarli, Macarena; Thompson, Carolina Soledad; Novoa, María Belen; Valentini, Beatriz Susana; Mastropaolo, Mariano; Echaide, Ignacio Eduardo; Torioni, Susana Marta; Primo, María Evangelina (American Association of Veterinary Laboratory Diagnosticians, 2019-11)Bovine anaplasmosis is a worldwide infectious disease caused by the intraerythrocytic bacterium Anaplasma marginale, which is transmitted by ticks and fomites. A. centrale is a less virulent subspecies used as a live ... -
Development and field evaluation of a nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) analysis to identify A. marginale-infected and A. centrale-vaccinated cattle
Primo, María Evangelina; Bellezze, Julio; Morel, Nicolas; Mazzucco Panizza, Matilde Nahimé; Valentini, Beatriz Susana; Torioni, Susana Marta; Thompson, Carolina Soledad (Elsevier, 2022-07)A nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) assay based on the amplification of the Anaplasma spp. highly conserved msp5 gene and posterior digestion with HindIII endonuclease ... -
Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle
Bellezze, Julio; Thompson, Carolina Soledad; Bosio, Anabela; Torioni, Susana Marta; Primo, María Evangelina (American Association of Veterinary Laboratory Diagnosticians, 2023-03)Immunization of calves with Anaplasma centrale is used to prevent acute anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is detected through serologic tests based primarily on A. marginale recombinant ... -
Development of a novel fusion protein with Anaplasma marginale and A. centrale MSP5 improved performance of Anaplasma antibody detection by cELISA in infected and vaccinated cattle
Primo, María Evangelina; Thompson, Carolina Soledad; Valentini, Beatriz Susana; Sarli, Macarena; Novoa, María Belen; Mangold, Atilio Jose; Torioni, Susana Marta (Plos One, 2019-01-23)Detection of antibodies to Anaplasma spp. using commercial competitive enzyme-linked immunosorbent assay (ccELISA) is based on the recombinant major surface protein 5 fused to maltose binding protein (MBP-MSP5) or glutathione ... -
Estudio de la diversidad genética en poblaciones de Babesia bigemina de diferentes regiones geográficas
Thompson, Carolina Soledad (Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 2013)La babesiosis bovina es una enfermedad enzoótica causada por protozoarios Apicomplexa del género Babesia. En Argentina, esta enfermedad es provocada por B. bovis y B. bigemina, ambas trasmitidas por la garrapata Rhipicephalus ... -
Ixodes silvanus n. sp. (Acari: Ixodidae), a new member of the subgenus Trichotoixodes Reznik, 1961 from northwestern Argentina
Saracho Bottero, Maria Noelia; Beati, Lorenza; Venzal, José Manuel; Guardia Claps, Leonor; Thompson, Carolina Soledad; Mangold, Atilio Jose; Guglielmone, Alberto; Nava, Santiago (Elsevier, 2020-09)Females, nymphs, and larvae of Ixodes silvanus n. sp. collected from birds and from the vegetation in northwestern Argentina (Yungas Phytogeographic Province) are described herein. The new species belongs to the subgenus ... -
Manual para el abordaje diagnóstico de la babesiosis y anaplasmosis bovina en Argentina
Mazzucco Panizza, Matilde Nahimé; Morel, Nicolas; Wilkowsky, Silvina Elizabeth; Primo, María Evangelina; Olmos, Leandro Hipolito; Pertile, Carla Natalia; Sarmiento, Nestor Fabian; Rossner, Maria Victoria; Abdala, Alejandro Ariel; Canton, German Jose; Ganzinelli, Sabrina Belen; Thompson, Carolina Soledad; Guillemi, Eliana Carolina; Nava, Santiago (Ediciones INTA, 2024-02)La babesiosis y la anaplasmosis bovina son dos enfermedades infecciosas causantes de importantes pérdidas económicas para la producción ganadera en el centro y norte de la Argentina, principalmente en concepto de mortandad ... -
Molecular detection and phylogenetic characterization of Theileria equi in horses (Equus caballus) from a peri-urban area of Argentina
Sebastian, Patrick Stephan; Benitez Ibalo, Alicia P.; Flores, Fernando Sebastian; Debárbora, Valeria Natalia; Martinez, Emilia Irina; Thompson, Carolina Soledad; Mangold, Atilio José (Elsevier, 2021-11)To investigate the presence of Theileria equi in an endemic area of equine piroplasmosis 42 horses (Equus caballus) from Corrientes City, Argentina were sampled. Eighty-one percent (34 blood samples) of the analyzed horses ... -
Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
Thompson, Carolina Soledad; Mangold, Atilio Jose; Félix, María L.; Carvalho, Luis A.; Armúa-Fernández, María Teresita; Venzal, José Manuel (2018-08)The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found ... -
Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
Martínez, Diana; Thompson, Carolina Soledad; Draghi, Maria Graciela; Canavesio, Vilma; Jacobo, Roberto; Zimmer, Patricia Andrea; Elena, Sebastián Alejandro; Nicola, Ana M.; Torioni, Susana Marta (2014-09-17)An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, ... -
Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
Primo, María Evangelina; Sarli, Macarena; Thompson, Carolina Soledad; Torioni, Susana Marta; Echaide, Ignacio Eduardo (Elsevier, 2021-09)The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem ... -
The Ixodes ricinus complex (Acari: Ixodidae) in the Southern Cone of America: Ixodes pararicinus, Ixodes aragaoi, and Ixodes sp. cf. I. affinis
Saracho Bottero, Maria Noelia; Venzal, José Manuel; Tarragona, Evelina Luisa; Thompson, Carolina Soledad; Mangold, Atilio Jose; Beati, Lorenza; Guglielmone, Alberto; Nava, Santiago (Springer, 2019-11)The goal of this study was to clarify the taxonomic status of the Ixodes ricinus complex in the Southern Cone of America, by using morphological characters and molecular markers (mitochondrial 16SrDNA and cox1 genes). The ... -
Typification of virulent and low virulence Babesia bigemina clones by 18S rRNA and rap-1c
Thompson, Carolina Soledad; Baravalle, María Eugenia; Valentini, Beatriz Susana; Mangold, Atilio Jose; Torioni, Susana Marta; Ruybal, Paula; Farber, Marisa Diana; Echaide, Ignacio Eduardo (2014-06)The population structure of original Babesia bigemina isolates and reference strains with a defined phenotypic profile was assessed using 18S rRNA and rap-1c genes. Two reference strains, BbiS2P-c (virulent) and BbiS1A-c ...