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resumen

Resumen
Cotton blue disease is the most important viral disease of cotton in the southern part of South America. Its etiological agent, cotton leafroll dwarf virus (CLRDV), is specifically transmitted to host plants by the aphid vector (Aphis gossypii) and any attempt to perform mechanical inoculations of this virus into its host has failed. This limitation has held back the study of this virus and the disease it causes. In this study, a full-length cDNA of CLRDV [ver mas...]
dc.contributor.authorDelfosse, Veronica Cecilia
dc.contributor.authorCasse, Maria Florencia
dc.contributor.authorAgrofoglio, Yamila Carla
dc.contributor.authorBonacic Kresic, Iván
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorZiegler-Graff, Véronique
dc.contributor.authorDistefano, Ana Julia
dc.date.accessioned2018-06-07T13:55:56Z
dc.date.available2018-06-07T13:55:56Z
dc.date.issued2013-07
dc.identifier.issn0168-1702
dc.identifier.otherhttps://doi.org/10.1016/j.virusres.2013.04.007
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S016817021300124X?via%3Dihub
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2565
dc.description.abstractCotton blue disease is the most important viral disease of cotton in the southern part of South America. Its etiological agent, cotton leafroll dwarf virus (CLRDV), is specifically transmitted to host plants by the aphid vector (Aphis gossypii) and any attempt to perform mechanical inoculations of this virus into its host has failed. This limitation has held back the study of this virus and the disease it causes. In this study, a full-length cDNA of CLRDV was constructed and expressed in vivo under the control of cauliflower mosaic virus 35S promoter. An agrobacterium-mediated inoculation system for the cloned cDNA construct of CLRDV was developed. Northern and immunoblot analyses showed that after several weeks the replicon of CLRDV delivered by Agrobacterium tumefaciens in Gossypium hirsutum plants gave rise to a systemic infection and typical blue disease symptoms correlated to the presence of viral RNA and P3 capsid protein. We also demonstrated that the virus that accumulated in the agroinfected plants was transmissible by the vector A. gossypii. This result confirms the production of biologically active transmissible virions. In addition, the clone was infectious in Nicotiana benthamiana plants which developed interveinal chlorosis three weeks postinoculation and CLRDV was detected both in the inoculated and systemic leaves. Attempts to agroinfect Arabidopsis thaliana plants were irregularly successful. Although no symptoms were observed, the P3 capsid protein as well as the genomic and subgenomic RNAs were irregularly detected in systemic leaves of some agroinfiltrated plants. The inefficient infection rate infers that A. thaliana is a poor host for CLRDV. This is the first report on the construction of a biologically-active infectious full-length clone of a cotton RNA virus showing successful agroinfection of host and non-host plants. The system herein developed will be useful to study CLRDV viral functions and plant–virus interactions using a reverse genetic approach.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVirus research 175 (1) : 64-70. (July 2013)es_AR
dc.subjectAlgodónes_AR
dc.subjectCottoneng
dc.subjectVirus de las Plantases_AR
dc.subjectPlant Viruseseng
dc.subjectADNes_AR
dc.subjectDNAeng
dc.subjectNicotianaes_AR
dc.subjectInoculaciónes_AR
dc.subjectInoculationeng
dc.subject.otherNicotiana benthamianaes_AR
dc.titleAgroinoculation of a full-length cDNA clone of cotton leafroll dwarf virus (CLRDV) results in systemic infection in cotton and the model plant Nicotiana benthamianaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Casse, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentinaes_AR
dc.description.filFil: Agrofoglio, Yamila Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Bonacic Kresic, Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Ziegler-Graff, Véronique. Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS conventionné avec l’Université de Strasbourg; Franciaes_AR
dc.description.filFil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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