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resumen

Resumen
Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated that cotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in [ver mas...]
dc.contributor.authorDelfosse, Veronica Cecilia
dc.contributor.authorAgrofoglio, Yamila Carla
dc.contributor.authorCasse, Maria Florencia
dc.contributor.authorBonacic Kresic, Iván
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorZiegler-Graff, Véronique
dc.contributor.authorDistefano, Ana Julia
dc.date.accessioned2018-06-07T12:19:12Z
dc.date.available2018-06-07T12:19:12Z
dc.date.issued2014-02
dc.identifier.issn0168-1702
dc.identifier.otherhttps://doi.org/10.1016/j.virusres.2013.12.018
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0168170213004474
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2564
dc.description.abstractPlants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated that cotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in Agrobacterium co-infiltration assay in Nicotiana benthamiana plants. A CLRDV full-length infectious cDNA clone that is able to infect N. benthamiana through Agrobacterium-mediated inoculation also inhibited local silencing in co-infiltration assays, suggesting that the P0 protein exhibits similar RNA silencing suppression activity when expressed from the full-length viral genome. On the other hand, the P0 protein did not efficiently inhibit the spread of systemic silencing signals. Moreover, Northern blotting indicated that the P0 protein inhibits the generation of secondary but not primary small interfering RNAs. The study of CLRDV P0 suppression activity may contribute to understanding the molecular mechanisms involved in the induction of cotton blue disease by CLRDV infection.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceVirus research 180 : 70-75. (February 2014)eng
dc.subjectVirus de las Plantases_AR
dc.subjectPlant Viruseseng
dc.subjectAlgodónes_AR
dc.subjectCottoneng
dc.subjectProteínases_AR
dc.subjectProteinseng
dc.subjectARNes_AR
dc.subjectRNAeng
dc.titleThe P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencinges_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Agrofoglio, Yamila Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Casse, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentinaes_AR
dc.description.filFil: Bonacic Kresic, Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Ziegler-Graff, Véronique. Institut de Biologie Moléculaire des Plantes, laboratoire propre du CNRS conventionné avec l’Université de Strasbourg; Franciaes_AR
dc.description.filFil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.subtypecientifico


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