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Abstract
Glycine-rich RNA-binding proteins (GR-RBPs) are involved in RNA processing and also some of them are output signals of the circadian clock. In tomato, one GR-RBP gene family (LeGRP1) is composed by three highly homologous genes (LeGRP1a-c); each one rendering three transcriptional products: the un-spliced pre-RNA (preLegrp1a-c), the mature mRNA (mLegrp1a-c) and the alternatively spliced mRNA (asLegrp1a-c). To get insight into their regulation and impact [ver mas...]
dc.contributor.authorRuggieri, Germán
dc.contributor.authorTriassi, Agustina
dc.contributor.authorAlvarez, Clarisa E.
dc.contributor.authorGola, Aldana
dc.contributor.authorWiggenhauser, Julieta
dc.contributor.authorBudde, Claudio Olaf
dc.contributor.authorLara, María Valeria
dc.contributor.authorMüller, Gabriela Leticia
dc.date.accessioned2018-05-17T17:34:15Z
dc.date.available2018-05-17T17:34:15Z
dc.date.issued2018
dc.identifier.issn1573-8264
dc.identifier.issn0006-3134
dc.identifier.otherhttps://doi.org/10.1007/s10535-018-0794-3
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs10535-018-0794-3
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2422
dc.description.abstractGlycine-rich RNA-binding proteins (GR-RBPs) are involved in RNA processing and also some of them are output signals of the circadian clock. In tomato, one GR-RBP gene family (LeGRP1) is composed by three highly homologous genes (LeGRP1a-c); each one rendering three transcriptional products: the un-spliced pre-RNA (preLegrp1a-c), the mature mRNA (mLegrp1a-c) and the alternatively spliced mRNA (asLegrp1a-c). To get insight into their regulation and impact on RNA metabolism in fruits, Solanum lycopersicum cv. Micro-Tom was transformed with preLeGRP1a fused to the polygalacturonase promoter, which drives expression to fruits from the mature green stage. Our results demonstrated a complex positive regulation of LeGRPs, in which LeGRP1a overexpression led to the induction of the others LeGRP1 members. Even though the LeGRP1 transcription and the content of three LeGRPs proteins were affected, the overall LeGRP protein circadian rhythm profile was similar in transgenic and WT fruits. However, when the fruits are kept at chilling temperature after harvest, total protein content was significantly higher in transgenic than in WT fruits, and the content of some free amino acids was modified. The results obtained suggest a probable role of LeGRP1s: structural rearrangements and/or stabilization of mRNA to allow efficient processing of fruits under cold conditions.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_AR
dc.sourceBiologia Plantarum 62 (3) : 501-510 (2018)es_AR
dc.subjectTomatees_AR
dc.subjectTomatoeseng
dc.subjectSolanum lycopersicumes_AR
dc.subjectAminoácidoses_AR
dc.subjectAmino Acidseng
dc.subjectRitmo Circadianoes_AR
dc.subjectCircadian Rhythmeng
dc.subjectPoligalacturonasaes_AR
dc.subjectPolygalacturonaseeng
dc.subjectARNes_AR
dc.subjectRNAeng
dc.subjectAlmacenamiento en fríoes_AR
dc.subjectCold Storageeng
dc.subjectProteínases_AR
dc.subjectProteinseng
dc.subject.otherLycopersicon esculentum
dc.titleOverexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storagees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.description.origenEEA San Pedroes_AR
dc.description.filFil: Ruggieri, German. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentinaes_AR
dc.description.filFil: Triassi, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentinaes_AR
dc.description.filFil: Álvarez, Clarisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentinaes_AR
dc.description.filFil: Gola, Aldana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentinaes_AR
dc.description.filFil: Wiggenhauser, Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentinaes_AR
dc.description.filFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentinaes_AR
dc.description.filFil: Lara, Maria Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentinaes_AR
dc.description.filFil: Drincovich, Maria F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentinaes_AR
dc.description.filFil: Müller, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentinaes_AR
dc.subtypecientifico


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