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Abstract
RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In this work, transgenic monoclonal cell lines constitutively expressing different amiRs targeting FMDV 3D-coding region or 3′UTR were established. Certain cell [ver mas...]
dc.contributor.authorGismondi, Maria Ines
dc.contributor.authorOrtiz, Xoana P.
dc.contributor.authorCurra, Anabella Paola
dc.contributor.authorAsurmendi, Sebastian
dc.contributor.authorTaboga, Oscar Alberto
dc.date.accessioned2018-04-19T13:27:48Z
dc.date.available2018-04-19T13:27:48Z
dc.date.issued2014-04
dc.identifier.issn0166-0934
dc.identifier.otherhttps://doi.org/10.1016/j.jviromet.2013.12.016
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0166093413005181#!
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2277
dc.description.abstractRNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In this work, transgenic monoclonal cell lines constitutively expressing different amiRs targeting FMDV 3D-coding region or 3′UTR were established. Certain cell lines showed an effective, sequence-specific amiR-mediated silencing activity that was accomplished by degradation of the target mRNA, as demonstrated in co-transfection experiments of reporter genes fused to FMDV target sequences. However, FMDV replication in these amiR-expressing cells was affected barely. Experiments aimed at elucidating the cause of RNAi failure demonstrated limited accessibility of the targeted region in the molecular environment of the viral RNA. Since RNAi is mediated by large-dimension silencing complexes containing the siRNA and not simply by a linear oligonucleotide, we propose that target selection should consider not only the local RNA structure but also the global conformation of target RNA.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceJournal of virological methods 199 : 1-10. (April 2014)eng
dc.subjectARNes_AR
dc.subjectRNAeng
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectVirus de las Plantases_AR
dc.subjectPlant Viruseseng
dc.subjectViricidases_AR
dc.subjectAntiviral Agentseng
dc.subject.otherMicroRNAes_AR
dc.subject.otherÁcido Ribonucléicoes_AR
dc.titleArtificial microRNAs as antiviral strategy to FMDV: structural implications of target selectioneng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Ortiz, Xoana P. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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