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BACKGROUND: Feral radish (Raphanus sativus L.) is a problematic weed that has become resistant to acetohydroxyacid synthase (AHAS) inhibitor herbicides due to the Trp574Leu mutation. An AHAS gene mutation that causes herbicide resistance may have negative pleiotropic effects on plant fitness. This study reports the effects of the Trp574Leu mutation on AHAS activity and reproductive traits of R. sativus. RESULTS: Eight of 17 feral radish accessions [ver mas...]
dc.contributor.authorVercellino, Roman B.
dc.contributor.authorPandolfo, Claudio Ezequiel
dc.contributor.authorBreccia, Gabriela
dc.contributor.authorCantamutto, Miguel Angel
dc.contributor.authorPresotto, Alejandro Daniel
dc.date.accessioned2018-04-05T15:50:41Z
dc.date.available2018-04-05T15:50:41Z
dc.date.issued2018-07
dc.identifier.issn1526-498X
dc.identifier.issn1526-4998
dc.identifier.otherhttps://doi.org/10.1002/ps.4849
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/abs/10.1002/ps.4849
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2180
dc.description.abstractBACKGROUND: Feral radish (Raphanus sativus L.) is a problematic weed that has become resistant to acetohydroxyacid synthase (AHAS) inhibitor herbicides due to the Trp574Leu mutation. An AHAS gene mutation that causes herbicide resistance may have negative pleiotropic effects on plant fitness. This study reports the effects of the Trp574Leu mutation on AHAS activity and reproductive traits of R. sativus. RESULTS: Eight of 17 feral radish accessions presented individuals resistant to metsulfuron‐methyl at 0.5% to >90.0% and all the resistant individuals analyzed showed the Trp574Leu mutation. Without herbicide selection, the AHAS activity was 3.2‐fold higher in the susceptible accession than in the resistant one. The resistant accession was >9000‐fold more resistant to metsulfuron‐methyl and imazethapyr than the susceptible accession. Under low intraspecific competition during two growing seasons, AHAS‐resistant feral radish accessions showed 22–38% and 21–47% lower seed numbers and yield per plant than the susceptible accession. CONCLUSION: This is the first report of fitness cost associated with the AHAS Trp574Leu mutation in R. sativus populations. This fitness cost could reduce frequency of the resistant allele without herbicide selectioneng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourcePest management science 74 (7):1600-1607. (2018 Jul.)eng
dc.subjectRaphanus sativuses_AR
dc.subjectActividad Enzimáticaes_AR
dc.subjectEnzyme Activityeng
dc.subjectGenética
dc.subjectGeneticseng
dc.subjectMutación
dc.subjectMutationeng
dc.titleAHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness costeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenEEA Hilario Ascasubies_AR
dc.description.filFil: Vercellino, Roman B. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentinaes_AR
dc.description.filFil: Pandolfo, Claudio Ezequiel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentinaes_AR
dc.description.filFil: Breccia, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cantamutto, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Hilario Ascasubi; Argentinaes_AR
dc.description.filFil: Presotto, Alejandro Daniel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentinaes_AR
dc.subtypecientifico


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