resumen
Abstract
We here explored the mechanisms associated with oxidative stress resistance and root colonization by soybean rhizobia. Genomic and physiological analyses supported the fact that the mutation of a highly conserved valine at position 263 to alanine in an aspartate kinase-like protein associated with oligopeptide uptake (LysC) enhances the oxidative stress resistance of the commercial inoculant Bradyrhizobium japonicum E109. Integrated genetic and
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dc.contributor.author | Brambilla, Silvina Maricel | |
dc.contributor.author | Liebrenz, Karen Ivana | |
dc.contributor.author | Frare, Romina Alejandra | |
dc.contributor.author | Gomez, Maria Cristina | |
dc.contributor.author | Stritzler, Margarita | |
dc.contributor.author | Soto, Gabriela Cynthia | |
dc.contributor.author | Ayub, Nicolás Daniel | |
dc.date | info:eu-repo/date/embargoEnd/2025-12-22 | |
dc.date.accessioned | 2024-08-22T10:48:31Z | |
dc.date.available | 2024-08-22T10:48:31Z | |
dc.date.issued | 2023-12 | |
dc.identifier.issn | 2452-2198 | |
dc.identifier.other | https://doi.org/10.1016/j.rhisph.2023.100823 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/19063 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/abs/pii/S2452219823001623 | |
dc.description.abstract | We here explored the mechanisms associated with oxidative stress resistance and root colonization by soybean rhizobia. Genomic and physiological analyses supported the fact that the mutation of a highly conserved valine at position 263 to alanine in an aspartate kinase-like protein associated with oligopeptide uptake (LysC) enhances the oxidative stress resistance of the commercial inoculant Bradyrhizobium japonicum E109. Integrated genetic and physiological studies suggest that LysC is part of a conserved substrate transport system in Bradyrhizobium, and that the LysC-V263A mutation in strain E109 significantly increased its oligopeptide uptake, survival during periods of starvation and nodulation competitiveness. Finally, the potential utilization of the CRISPR system to promptly propagate this gain-of-function mutation in soybean production worldwide is discussed. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Elsevier | es_AR |
dc.rights | info:eu-repo/semantics/embargoedAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_AR |
dc.source | Rhizosphere 28 : 100823 (December 2023) | es_AR |
dc.subject | Oligopeptides | eng |
dc.subject | Oligopéptidos | es_AR |
dc.subject | Oxidative Stress | eng |
dc.subject | Estrés Oxidativo | es_AR |
dc.subject | Soybeans | eng |
dc.subject | Soja | es_AR |
dc.subject | Rhizobiaceae | eng |
dc.subject | Bradyrhizobium japonicum | eng |
dc.subject | CRISPR | eng |
dc.subject | Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas | es_AR |
dc.subject.other | Inoculants | eng |
dc.subject.other | Inoculantes | es_AR |
dc.subject.other | Oxidative Stress Resistance | eng |
dc.subject.other | Resistencia al Estrés Oxidativo | es_AR |
dc.title | Oligopeptide uptake improves oxidative stress resistance and nodulation competitiveness in soybean rhizobia | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/acceptedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | es_AR |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Liebrenz, Karen Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Liebrenz, Karen Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.subtype | cientifico |
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