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resumen

Resumen
The storage of peaches at low temperatures for prolonged periods can induce a form of chilling injury (CI) called woolliness, characterized by a lack of juiciness and a mealy texture. As this disorder has been associated with abnormal cell wall dismantling, the levels of 12 transcripts encoding proteins involved in cell wall metabolism were analysed in cultivars with contrasting susceptibility to this disorder selected from five melting flesh peach [ver mas...]
dc.contributor.authorGenero, Melisa
dc.contributor.authorGismondi, Mauro
dc.contributor.authorMonti, Laura
dc.contributor.authorGabilondo, Julieta
dc.contributor.authorBudde, Claudio Olaf
dc.contributor.authorAndreo, Carlos Santiago
dc.contributor.authorLara, María Valeria
dc.contributor.authorDrincovich, María Fabiana
dc.contributor.authorBustamante, Claudia Anabel
dc.date.accessioned2017-12-06T13:49:30Z
dc.date.available2017-12-06T13:49:30Z
dc.date.issued2016-02-23
dc.identifier.issn1432-203X
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1778
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00299-016-1956-4
dc.description.abstractThe storage of peaches at low temperatures for prolonged periods can induce a form of chilling injury (CI) called woolliness, characterized by a lack of juiciness and a mealy texture. As this disorder has been associated with abnormal cell wall dismantling, the levels of 12 transcripts encoding proteins involved in cell wall metabolism were analysed in cultivars with contrasting susceptibility to this disorder selected from five melting flesh peach cultivars. The resistant (‘Springlady’) and susceptible (‘Flordaking’) cultivars displayed differences in the level of expression of some of the selected genes during fruit softening and in woolly versus non-woolly fruits. From these genes, the level of expression of PpXyl, which encodes for a putative β-xylosidase, was the one that presented the highest correlation (negative) with the susceptibility to woolliness. PpXyl expression was also analysed in a cultivar (‘Rojo 2’) with intermediate susceptibility to woolliness, reinforcing the conclusion about the correlation of PpXyl expression to the presence of woolliness symptom. Moreover, the level of expression of PpXyl correlated to protein level detected by Western blot. Analyses of the promoter region of the PpXyl gene (1637 bp) isolated from the three cultivars showed no differences suggesting that cis-elements from other regions of the genome and/or trans elements could be responsible of the differential PpXyl expression patterns. Overall, the results obtained indicate that PpXyl may act as a good indicator of woolliness tolerance and that the regulation of expression of this gene in different cultivars does not depend on sequences upstream the coding sequence.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourcePlant cell reports 35 (6) : 1235–1246. (June 2016)
dc.subjectDuraznoes_AR
dc.subjectPeacheses_AR
dc.subjectPrunus persicaes_AR
dc.subjectAlmacenamiento en Fríoes_AR
dc.subjectCold Storagees_AR
dc.subjectMetabolismoes_AR
dc.subjectMetabolismes_AR
dc.subjectFisiología ´Postcosechaes_AR
dc.subjectPostharvest Physiologyes_AR
dc.titleCell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerancees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenEEA San Pedroes_AR
dc.description.filFil: Genero, Marisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentinaes_AR
dc.description.filFil: Gismondi, Mauro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentinaes_AR
dc.description.filFil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentinaes_AR
dc.description.filFil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentinaes_AR
dc.description.filFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro San Pedro; Argentinaes_AR
dc.description.filFil: Andreo, Carlos Santiago. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentinaes_AR
dc.description.filFil: Lara, María V.Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentinaes_AR
dc.description.filFil: Drincovich, María Fabiana .Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentinaes_AR
dc.description.filFil: Bustamante, Claudia A.Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentinaes_AR
dc.subtypecientifico


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