Facebook
Twitter
YouTube
Instagram
    • español
    • English
  • Contacto
  • español 
    • español
    • English
  • Mi Cuenta
Acerca deAutoresTítulosTemasColeccionesComunidades☰
JavaScript is disabled for your browser. Some features of this site may not work without it.
Ver ítem 
    xmlui.general.dspace_homeCentros e Institutos de InvestigaciónCIAP. Centro de Investigaciones AgropecuariasInstituto de Patología VegetalArtículos científicosxmlui.ArtifactBrowser.ItemViewer.trail
  • Inicio
  • Centros e Institutos de Investigación
  • CIAP. Centro de Investigaciones Agropecuarias
  • Instituto de Patología Vegetal
  • Artículos científicos
  • Ver ítem

Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina

Resumen
Strawberry (Fragaria x ananassa Duch. cv. Camino Real) plants showing phyllody symptoms were detected in production fields in Bella Vista (Corrientes province, Argentina). DNA from all symptomatic samples analyzed yielded fragments of the expected size in PCR reactions using phytoplasma universal primers. DNA from asymptomatic plants yielded no products. The associated phytoplasma was designated as Argentinean Strawberry Phyllody (ASP). The ASP sequence [ver mas...]
Strawberry (Fragaria x ananassa Duch. cv. Camino Real) plants showing phyllody symptoms were detected in production fields in Bella Vista (Corrientes province, Argentina). DNA from all symptomatic samples analyzed yielded fragments of the expected size in PCR reactions using phytoplasma universal primers. DNA from asymptomatic plants yielded no products. The associated phytoplasma was designated as Argentinean Strawberry Phyllody (ASP). The ASP sequence of the 16S rRNA gene showed 99–98% homology with members of the 16SrVII ash yellows group. The putative RFLP profile was indistinguishable from the Argentinean alfalfa witches’ broom (ArAWB) phytoplasma. The phylogenetic analysis of nearly full-length 16S rDNA sequence and 16S-23S spacer region yielded a consensus tree wherein ASP clustered into the ash yellows group (16Sr VII) with high confidence values (95), generating a separated branch (100 bootstrap value) together with ArAWB (16Sr VII-C). In Argentina, the 16Sr VII group was also detected in alfalfa (Medicago sativa L.) crops, and weeds [Artemisa annua L. and Conyza bonariensis (L.) Cronquist]. To our knowledge, this is the first report of a phytoplasma from the 16Sr VII group affecting strawberries in Argentina and worldwide [Cerrar]
Thumbnail
Autor
Fernandez, Franco Daniel;   Conci, Vilma Cecilia;   Kirschbaum, Daniel Santiago;   Conci, Luis Rogelio;  
Fuente
European journal of plant pathology 135 (1) : 1–4. (January 2013)
Fecha
2013-01
ISSN
0929-1873 (Print)
1573-8469 (Online)
URI
http://hdl.handle.net/20.500.12123/1727
https://link.springer.com/article/10.1007/s10658-012-9951-2
DOI
https://doi.org/10.1007/s10658-012-9951-2
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Fresa; Strawberries; Fitoplasmas; Phytoplasmas; Biología Molecular; Molecular Biology; Frutilla; Argentina;
Derechos de acceso
Restringido
Descargar
Compartir
  • Compartir
    Facebook Email Twitter Mendeley
Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Metadatos
Mostrar el registro completo del ítem