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Abstract
Strawberry (Fragaria x ananassa Duch. cv. Camino Real) plants showing phyllody symptoms were detected in production fields in Bella Vista (Corrientes province, Argentina). DNA from all symptomatic samples analyzed yielded fragments of the expected size in PCR reactions using phytoplasma universal primers. DNA from asymptomatic plants yielded no products. The associated phytoplasma was designated as Argentinean Strawberry Phyllody (ASP). The ASP sequence [ver mas...]
dc.contributor.authorFernandez, Franco Daniel
dc.contributor.authorConci, Vilma Cecilia
dc.contributor.authorKirschbaum, Daniel Santiago
dc.contributor.authorConci, Luis Rogelio
dc.coverage.spatialArgentina (nation)
dc.date.accessioned2017-11-09T13:20:48Z
dc.date.available2017-11-09T13:20:48Z
dc.date.issued2013-01
dc.identifier.issn0929-1873 (Print)
dc.identifier.issn1573-8469 (Online)
dc.identifier.otherhttps://doi.org/10.1007/s10658-012-9951-2
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1727
dc.identifier.urihttps://link.springer.com/article/10.1007/s10658-012-9951-2
dc.description.abstractStrawberry (Fragaria x ananassa Duch. cv. Camino Real) plants showing phyllody symptoms were detected in production fields in Bella Vista (Corrientes province, Argentina). DNA from all symptomatic samples analyzed yielded fragments of the expected size in PCR reactions using phytoplasma universal primers. DNA from asymptomatic plants yielded no products. The associated phytoplasma was designated as Argentinean Strawberry Phyllody (ASP). The ASP sequence of the 16S rRNA gene showed 99–98% homology with members of the 16SrVII ash yellows group. The putative RFLP profile was indistinguishable from the Argentinean alfalfa witches’ broom (ArAWB) phytoplasma. The phylogenetic analysis of nearly full-length 16S rDNA sequence and 16S-23S spacer region yielded a consensus tree wherein ASP clustered into the ash yellows group (16Sr VII) with high confidence values (95), generating a separated branch (100 bootstrap value) together with ArAWB (16Sr VII-C). In Argentina, the 16Sr VII group was also detected in alfalfa (Medicago sativa L.) crops, and weeds [Artemisa annua L. and Conyza bonariensis (L.) Cronquist]. To our knowledge, this is the first report of a phytoplasma from the 16Sr VII group affecting strawberries in Argentina and worldwideeng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceEuropean journal of plant pathology 135 (1) : 1–4. (January 2013)eng
dc.subjectFresaes_AR
dc.subjectStrawberrieseng
dc.subjectFitoplasmases_AR
dc.subjectPhytoplasmaseng
dc.subjectBiología Moleculares_AR
dc.subjectMolecular Biologyeng
dc.subject.otherFrutilla
dc.subject.otherArgentina
dc.titleMolecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentinaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.filFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
dc.description.filFil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Kirschbaum, Daniel Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
dc.description.filFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba; Argentina
dc.subtypecientifico


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