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Mostrando ítems 1-20 de 21
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A thermostable GH8 endoglucanase of Enterobacter sp. R1 is suitable for β- glucan deconstruction
Ontañon, Ornella Mailen; Ghio, Silvina; Marrero Diaz De Villegas, Rubén; Garrido, Mercedes Maria; Talia, Paola Mónica; Fehér, Csaba; Campos, Eleonora (Elsevier, 2019-11)Glycoside hydrolase family 8 (GH8) includes endoglucanases, lichenases, chitosanases and xylanases, which are essential for polysaccharides breakdown. In this work, we studied a thermally stable GH8 from the cellulose ... -
Characterization of a novel GH10 alkali‑thermostable xylanase from a termite microbiome
Mon, Maria Laura; Marrero Diaz De Vill, Rubén; Campos, Eleonora; Soria, Marcelo Abel; Talia, Paola Mónica (Springer Open, 2022-08)The aim of the present study was to assess the biochemical and molecular structural characteristics of a novel alkali-thermostable GH10 xylanase (Xyl10B) identified in a termite gut microbiome by a shotgun metagenomic ... -
Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
Romano, Nelson Gaston; Gioffre, Andrea; Sede, Silvana Mabel; Campos, Eleonora; Cataldi, Angel Adrian; Talia, Paola Mónica (Springer, 2013-08)Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups ... -
Characterization of two GH5 endoglucanases from termite microbiome using synthetic metagenomics
Ben Guerrero, Emiliano; Marrero Diaz De Villegas, Ruben; Soria, Marcelo Abel; Santangelo, María De La Paz; Campos, Eleonora; Talia, Paola Mónica (Springer, 2020-08)Here, we characterize two novel GH5 endoglucanases (GH5CelA and GH5CelB) from an uncultured bacterium identified in termite gut microbiomes. Both genes were codon-optimized, synthetized, cloned, and expressed as recombinant ... -
Draft Genome Sequence of Cellulolytic and Xylanolytic Cellulomonas sp. Strain B6 Isolated from Subtropical Forest Soil
Piccinni, Florencia Elizabeth; Murua, Yanina; Ghio, Silvina; Talia, Paola Mónica; Rivarola, Maximo Lisandro; Campos, Eleonora (2016-08)The genome information will be useful for studies of microbial enzymes for industrial application in lignocellulosic biomass utilization. -
EcXyl43 β-xylosidase: molecular modeling, activity on natural and artificial substrates, and synergism with endoxylanases for lignocellulose deconstruction
Ontañon, Ornella Mailén; Ghio, Silvina; Marrero Diaz De Vill, Rubén; Piccinni, Florencia Elizabeth; Talia, Paola Mónica; Cerutti, Maria Laura; Campos, Eleonora (Springer, 2018-08)Biomass hydrolysis constitutes a bottleneck for the biotransformation of lignocellulosic residues into bioethanol and high-value products. The efficient deconstruction of polysaccharides to fermentable sugars requires ... -
Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
Capetti, Caio; Arnoldi Pellegrini, Vanessa; Vacilotto, Milena Moreira; da Silva Curvelo, Antonio Aprigio; Falvo, Maurício; Guimaraes, Francisco E.G.; Ontañon, Ornella Mailen; Campos, Eleonora; Polikarpov, Igor (Springer, 2023-10)Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are ... -
Heterologous production and characterization of a thermostable GH10 family endo-xylanase from Pycnoporus sanguineus BAFC 2126
Niderhaus, Cecilia; Garrido, Mercedes Maria; Insani, Ester Marina; Campos, Eleonora; Wirth, Sonia Alejandra (Elsevier, 2018-04)Xylanases are key enzymes for agricultural biomass saccharification for the production of cellulosic ethanol. Success in enzymatic lignocellulose bioconversion is restricted by enzyme production costs, activity and stability ... -
Insights into the xylan degradation system of Cellulomonas sp. B6 : biochemical characterization of rCsXyn10A and rCsAbf62A
Garrido, Mercedes Maria; Piccinni, Florencia Elizabeth; Landoni, Malena; Peña, María Jesús; Topalian, Juliana; Couto, Alicia; Wirth, Sonia Alejandra; Urbanowicz, Breeanna Rae; Campos, Eleonora (Springer, 2022)Valorization of the hemicellulose fraction of plant biomass is crucial for the sustainability of lignocellulosic biorefineries. The Cellulomonas genus comprises Gram-positive Actinobacteria that degrade cellulose and other ... -
Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
Romero Victorica, Matias; Soria, Marcelo Abel; Batista-García, Ramón Alberto; Ceja-Navarro, Javier A.; Vikram, Surendra; Ortiz, Maximiliano; Ontañon, Ornella; Ghio, Silvina; Martínez-Ávila, Liliana; Quintero García, Omar Jasiel; Etcheverry, Clara; Campos, Eleonora; Cowan, Donald; Arneodo Larochette, Joel Demian; Talia, Paola Mónica (Springer Nature, 2020-03-02)In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different ... -
Obtención de mutantes de Brucella abortus genéticamente definidas: su caracteización molecular y evaluación de su uso como vacunas para la brucelosis bovina
Campos, Eleonora (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2017)El desarrollo de las técnicas de ADN recombinante permitió la generación de vacunas racionalmente diseñadas. Teniendo esto en cuenta, el objetivo de este trabajo es la obtención de cepas de Brucella abortus genéticamente ... -
Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
Ontañon, Ornella Mailen; Bedő, Soma; Ghio, Silvina; Garrido, Mercedes Maria; Topalian, Juliana; Jahola, Dóra; Fehér, Anikó; Valacco, Maria Pia; Campos, Eleonora; Fehér, Csaba (Springer, 2021-05)One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still ... -
Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
Coniglio, Romina O.; Fonseca, María I.; Diaz, Gabriela V.; Ontañon, Ornella Mailén; Ghio, Silvina; Campos, Eleonora; Zapata, Pedro Darío (Taylor & Francis, 2019-04)The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing ... -
Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil
Ghio, Silvina; Sauka, Diego Hernan; Ferrari, Alejandro E.; Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Campos, Eleonora (Microbiology Society, 2019-12)A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina. Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium. Its ... -
Prospección y caracterización de bacterias celulolíticas y hemicelulolíticas
Ghio, Silvina (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2018)La prospección de bacterias con capacidad de degradar lignocelulosa permite la identificación de nuevas enzimas con potencial aplicación en numerosos procesos industriales. En este trabajo el objetivo fue la obtención de ... -
PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus
Garrido, Mercedes Maria; Landoni, Malena; Sabbadin, Federico; Valacco, María Pía; Couto, Alicia; Bruce, Neil Charles; Wirth, Sonia Alejandra; Campos, Eleonora (Springer, 2020-09)Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a ... -
Purification and characterization of a GH43 β-xylosidase from Enterobacter sp. identified and cloned from forest soil bacteria
Campos, Eleonora; Negro Alvarez, María José; Sabaris Di Lorenzo, Gonzalo Julián; Gonzalez, Sergio Alberto; Rorig, Marcela Laura; Talia, Paola Mónica; Grasso, Daniel Horacio; Sáez, Felicia; Manzanares Secades, Paloma; Ballesteros Perdices, Mercedes; Cataldi, Angel Adrian (Elsevier, 2014-03)The use of lignocellulosic biomass for second generation biofuels requires optimization of enzymatic breakdown of plant cell walls. In this work, cellulolytic bacteria were isolated from a native and two cultivated forest ... -
Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Ghio, Silvina; Sauka, Diego Hernan; Talia, Paola Mónica; Rivarola, Maximo Lisandro; Valacco, María Pía; Campos, Eleonora (Wiley; Society for Applied Microbiology, 2018-12)Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual ... -
Structural and molecular dynamics investigations of ligand stabilization via secondary binding site interactions in Paenibacillus xylanivorans GH11 xylanase
Briganti, Lorenzo; Capetti, Caio; Pellegrini, Vanessa O. A.; Ghio, Silvina; Campos, Eleonora; Nascimento, Alessandro S.; Polikarpov, Igor (Elsevier, 2021-03)Glycoside hydrolases (GHs) are essential for plant biomass deconstruction. GH11 family consist of endo-β-1,4-xylanases which hydrolyze xylan, the second most abundant cell wall biopolymer after cellulose, into small ... -
Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversio
Ghio, Silvina; Bradanini, María B.; Garrido, Mercedes Maria; Ontañon, Ornella Mailen; Piccinni, Florencia Elizabeth; Marrero Diaz De Vill, Rubén; Talia, Paola Mónica; Campos, Eleonora (Elsevier, 2020-12)In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase ...