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Mostrando ítems 1-5 de 5
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A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
Trangoni, Marcos David; Gioffre, Andrea; Cravero, Silvio Lorenzo Pedro (Springer, 2017-06)LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, ... -
Campylobacter fetus releases S-layered and immunoreactive outer membrane vesicles = Campylobacter fetus secreta vesículas de membrana externa inmunorreactivas que conservan la capa S
Farace, Pablo; Cravero, Silvio Lorenzo Pedro; Taibo, Catalina Beatriz; Diodati, Julian Angel; Morsella, Claudia Graciela; Paolicchi, Fernando; Sabio Y Garcia, Julia Veronica; Gioffre, Andrea (Elsevier, 2021-08)The study of outer membrane vesicles (OMVs) became relevant because of their probable important role in the transfer of virulence factors to host cells. Campylobacter fetus is mainly a mammal pathogen whose virulence ... -
Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex
Avila, Héctor Gabriel; Mozzoni, Cecilia; Trangoni, Marcos David; Cravero, Silvio Lorenzo Pedro; Pérez, Verónica Mirtha; Valenzuela, Federico; Gertiser, María Laura; Butti, Marcos Javier; Kamenetzky, Laura; Jensen, Oscar; Rosenzvit, Mara Cecilia (Elsevier, 2020-01)Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is ... -
L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus
Farace, Pablo Daniel; Morsella, Claudia Graciela; Cravero, Silvio Lorenzo Pedro; Sioya, Bernardo Arturo; Amadio, Ariel; Paolicchi, Fernando; Gioffre, Andrea (2019-11)Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive ... -
LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
Trangoni, Marcos David; Gioffre, Andrea; Ceron Cucchi, Maria Esperanza; Caimi, Karina Cynthia; Ruybal, Paula; Zumarraga, Martin Jose; Cravero, Silvio Lorenzo Pedro (Sociedade Brasileira de Microbiologia, 2015-06)In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific ...