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A beginner’s guide for FMDV quasispecies analysis: sub-consensus variant detection and haplotype reconstruction using next-generation sequencing
(Oxford Academic Press, 2020-09)Deep sequencing of viral genomes is a powerful tool to study RNA virus complexity. However, the analysis of next-generation sequencing data might be challenging for researchers who have never approached the study of viral ... -
A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit
(Society for Experimental Biology, 2008-07)In tomato, numerous wild-related species have been demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of ... -
A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
(Springer, 2017-06)LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, ... -
A comprehensive analysis of the genetic diversity and environmental adaptability in worldwide Merino and Merino‑derived sheep breeds
(BioMed Central, 2023-04)Background: To enhance and extend the knowledge about the global historical and phylogenetic relationships between Merino and Merino-derived breeds, 19 populations were genotyped with the OvineSNP50 BeadChip specifically ... -
A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies
(Elsevier, 2015-10)Foot and mouth disease is caused by a non-enveloped virus (FMDV), which disposes several antigenic sites at the surface of their capsid proteins. The most relevant and immunodominant antigenic site of FMDV (site A or AnSA) ... -
A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
(Nature Publishing Group, 2017-12)The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that ... -
A direct high-throughput in Cell-ELISA for measuring infectivity of cytopathic and non-cytopathic bovine viral diarrhoea virus strains applied to the assessment of antiviral activity
(Elsevier, 2018-07-19)Low-cost high-throughput methods applicable to any virus strain are required for screening antiviral compounds against multiple field strains. Colorimetric cell-viability assays are used for this purpose as long as the ... -
A DNA vaccine formulated with chemical adjuvant provides partial protection against bovine herpes virus infection in cattle
(2017-01-25)Bovine herpesvirus-1 (BoHV-1) is the causative agent of bovine infectious rhinotracheitis, an important disease worldwide. Although conventional BoHV-1 vaccines, including those based on the use of modified live virus and ... -
A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers
(American Society for Microbiology, 2023-02)Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein ... -
A fungal protease named AsES triggers antiviral immune responses and effectively restricts virus infection in arabidopsis and Nicotiana benthamiana plants
(Oxford University Press, 2022-04)Background and Aims: Plants have evolved complex mechanisms to fight against pathogens. Among these mechanisms, pattern-triggered immunity (PTI) relies on the recognition of conserved microbe- or pathogen-associated molecular ... -
A look at polyploidy and plant breeding
(Heighten Science Publications, 2022-11)Polyploidization is a process that generates genetic variability and therefore one of the engines of biological evolution. Since polyploidization produces important changes in the phenotype, mainly an increase in the size ... -
A method for semi-field rearing of Varroa destructor (Acari: Varroidae) to obtain mites of controlled age and specific life cycle
(Les Amis d'Acarologia, 2023-03)Varroa destructor is one of the most devastating ectoparasites of the honey bee, Apis mellifera, worldwide. Given that V. destructor has very low survivability and exhibits no successful reproduction away from their natural ... -
A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine
(Frontiers Media, 2020-07)Foot-and-Mouth Disease (FMD) is an acute viral disease that causes important economy losses. Vaccines with new low-cost adjuvants that stimulate protective immune responses are needed and can be assayed in a mouse model ... -
A new gnotobiotic pig model of P[6] human rotavirus infection and disease for preclinical evaluation of rotavirus vaccines
(MDPI, 2022-12)Human rotavirus (HRV) is a leading cause of gastroenteritis in children under 5 years of age. Licensed vaccines containing G1P[8] and G1-4P[8] strains are less efficacious against newly emerging P[6] strains, indicating ... -
A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
(Sage Publications, 2020-02)Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization ... -
A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
(BMC, 2020-07)Background: Neonatal diarrhea remains one of the main causes of morbi-mortality in dairy calves under artificial rearing. It is often caused by infectious agents of viral, bacterial, or parasitic origin. Cows vaccination ... -
A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp.
(BMC, 2020-04)Introduction: Babesiosis is a protozoan tick-borne infection associated with anemia and life-threatening disease in humans, domestic and wildlife animals. Dogs are infected by at least six well-characterized Babesia spp. ... -
A new variety of Solanum sisymbriifolium obtained by in vitro polyploidization
(Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, 2024)Solanum sisymbriifolium is a shrub native to South America and considered a weed of several important crops. Despite this, this species has proven nematicidal capacity and medicinal properties, due to its ability to produce ... -
A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads
(2017)Bovine leukemia virus (BLV) is associated with the most common neoplastic disease of cattle. BLV has a silent dissemination in the herd due to infected cell exchange, thus the concentration of BLV-infected cells in blood ... -
A novel bacterial protease inhibitor adjuvant in RBD-based COVID-19 vaccine formulations containing alum increases neutralizing antibodies, specific germinal center B cells and confers protection against SARS-CoV-2 infection in mice
(Frontiers Media, 2022-02)In this work, we evaluated recombinant receptor binding domain (RBD)-based vaccine formulation prototypes with potential for further clinical development. We assessed different formulations containing RBD plus alum, AddaS03, ...